郜海燕,肖尚月,陈杭君,黎云龙,刘瑞玲,吴伟杰.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究[J].农业机械学报,2017,48(5):327-334.
GAO Haiyan,XIAO Shangyue,CHEN Hangjun,LI Yunlong,LIU Ruiling,WU Weijie.Identification and Biological Characteristics of Fungal Pathogens in Blueberry during Postharvest Storage[J].Transactions of the Chinese Society for Agricultural Machinery,2017,48(5):327-334.
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蓝莓采后主要病原真菌的分离鉴定与生物学特性研究   [下载全文]
Identification and Biological Characteristics of Fungal Pathogens in Blueberry during Postharvest Storage   [Download Pdf][in English]
投稿时间:2017-02-13  
DOI:10.6041/j.issn.1000-1298.2017.05.041
中文关键词:  蓝莓  采后病害  分离鉴定  rDNA-ITS  生物学特性
基金项目:国家自然科学基金项目(31601523)、公益性行业(农业)科研专项(201303073)和浙江省重大科技专项重点农业项目(2014C02024)
作者单位
郜海燕 浙江省农业科学院 
肖尚月 浙江省农业科学院 
陈杭君 浙江省农业科学院 
黎云龙 浙江省农业科学院 
刘瑞玲 浙江省农业科学院 
吴伟杰 浙江省农业科学院 
中文摘要:霉变是导致蓝莓采后损失、货架期短的主要原因之一,为确定蓝莓贮藏期的主要致腐病原真菌,对浙江省杭州市安吉县蓝莓产区发病果实的病原菌进行分离纯化,得到5株病原真菌(LMA、LMB、LMC1、LMC2和LMC3)。通过形态学和rDNA-ITS序列分析,初步鉴定为B. cinerea、T. viridescens、P. cecidicola、P. polonicum和P. expansum。分离菌株的生物学特性研究表明,5株病原菌适宜生长条件为:蓝莓汁—葡萄糖培养基,25~30℃;菌株LMA、LMC1 和LMC2的最适碳源为果糖,菌株LMB和菌株LMC3的最适碳源分别为蔗糖和可溶性淀粉;菌株LMA、LMC1 和LMC2的最适氮源为蛋白胨,菌株LMB和LMC3的最适氮源为酵母浸出粉。光照对菌株LMA、LMC1和LMC2病原菌菌丝生长有明显影响,而对菌株LMB和LMC3影响较小。
GAO Haiyan  XIAO Shangyue  CHEN Hangjun  LI Yunlong  LIU Ruiling  WU Weijie
Zhejiang Academy of Agricultural Science,Zhejiang Academy of Agricultural Science,Zhejiang Academy of Agricultural Science,Zhejiang Academy of Agricultural Science,Zhejiang Academy of Agricultural Science and Zhejiang Academy of Agricultural Science
Key Words:blueberry  postharvest disease  isolation and identification  rDNA-ITS  biological characteristics
Abstract:Mold rot is one of the main factors which lead to the postharvest loss and short shelf-life of blueberry fruits. The objective of this study is to determine the primary fungal pathogens which may cause postharvest decay of blueberry during storage process, and to investigate the biological characteristics of pathogens, providing theoretical basis for disease control and further research. The typical postharvest decay sympotoms of blueberry were described. Five strains designated as LMA, LMB, LMC1, LMC2 and LMC3 were respectively isolated from diseased blueberry fruit harvested in Anji County of Zhejiang Province. Isolates were characterized by colony morphology and conidial characteristics at 28℃ in dark. The internal transcribed spacer (ITS) region of the ribosomal DNA (ITS1/ITS4) from five isolates was amplified and sequenced. The consensus ITS sequences of five isolates were compared with the associated sequences in GenBank. MEGA 5.0 was used to draw the phylogenetic tree of five isolates and other related sequences. The isolated strains were identified as B. cinerea, T. viridescens, P. cecidicola, P. polonicum and P. expansum, respectively, according to the morphologies of colony and conidial and the rDNA-ITS sequence analysis. The biological characteristics of the isolated strains were further investigated including the effects of different medias, carbon and nitrogen sources, temperatures, pH values and light on mycelial growth of five fungal pathogens using cross method, and the results showed that the suitable culture media and temperature for the five strains were blueberry extract-glucose media and 25~30℃, respectively. The optimal carbon source was fructose for LMA, LMC1 and LMC2 strains, sucrose for LMB strain, and soluble starch for LMC3 strain. The optimal nitrogen source was peptone for LMA, LMC1 and LMC2 strains, yeast extract powder for LMB1 and LMC3 strains. The effects of light on the mycelia growth of LMA, LMC1 and LMC2 were significant, but that was insignificant on the growth of LMB and LMC3 strains.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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