左惠心,韩玲,余群力,牛克兰,赵索南,孔祥颖.牦牛与黄牛肌肉差异蛋白质组及生物信息学分析[J].农业机械学报,2017,48(4):313-320.
ZUO Huixin,HAN Ling,YU Qunli,NIU Kelan,ZHAO Suonan,KONG Xiangying.Proteomics and Bioinformatics Analyses of Differentially Expressed Proteins in Yak and Beef Cattle Muscle[J].Transactions of the Chinese Society for Agricultural Machinery,2017,48(4):313-320.
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牦牛与黄牛肌肉差异蛋白质组及生物信息学分析   [下载全文]
Proteomics and Bioinformatics Analyses of Differentially Expressed Proteins in Yak and Beef Cattle Muscle   [Download Pdf][in English]
投稿时间:2017-02-14  
DOI:10.6041/j.issn.1000-1298.2017.04.041
中文关键词:  牦牛  黄牛  肌肉组织  双向电泳  差异蛋白质组学  生物信息学
基金项目:国家自然科学基金项目(31460402)和国家现代农业产业(肉牛牦牛)技术体系项目(CARS-38)
作者单位
左惠心 甘肃农业大学 
韩玲 甘肃农业大学 
余群力 甘肃农业大学 
牛克兰 甘肃农业大学 
赵索南 海北州畜牧兽医科学研究所 
孔祥颖 海北州畜牧兽医科学研究所 
中文摘要:为了建立和优化牦牛肌肉组织蛋白质双向电泳(2DE)体系,结合生物信息学方法进行牦牛、黄牛差异蛋白质通路分析。以牦牛背最长肌为实验材料,对不同裂解液成分、等电聚焦程序、染色方法进行研究,在最优2DE体系参数下,对比分析牦牛、黄牛差异倍数大于2倍且达到显著水平(P<0.05)的19个蛋白质,通过基质辅助激光解吸/电离飞行时间(MALDI-TOF/TOF)质谱进行鉴定,并对鉴定结果进行了基因本体(GO)注释、京都基因与基因组百科全书(KEGG)通路分析。结果表明,裂解液II、渐进式快速升压程序、改良的考染法获得的蛋白点匹配率高,牦牛、黄牛2DE图谱蛋白点平均个数分别为479个和553个。通过比较牦牛和黄牛背最长肌中差异蛋白质可知,所得到的差异蛋白质按照功能可分为代谢酶、结构蛋白和应激蛋白3大类。通过KEGG分析可知,牦牛、黄牛差异蛋白质主要集中在细胞代谢过程、碳水化合物代谢通路、遗传信息通路和能量代谢通路中,研究结果可为解释牦牛和黄牛肌肉生物学特性和肉品质差异提供理论依据。
ZUO Huixin  HAN Ling  YU Qunli  NIU Kelan  ZHAO Suonan  KONG Xiangying
Gansu Agricultural University,Gansu Agricultural University,Gansu Agricultural University,Gansu Agricultural University,Institute of Animal and Veterinary Science of Haibei Tibetan Autonomous Prefecture and Institute of Animal and Veterinary Science of Haibei Tibetan Autonomous Prefecture
Key Words:yak  beef cattle  muscle tissue  two dimensional electrophoresis  differential proteomics  bioinformatics
Abstract:Yak (Bos grunniens) lives at plateau area of more than 3500 altitude meter, in this case, yak still maintains normal physiological activity. Besides, yak meat is rich in protein and low in fat, which does not contain anabolic steroids. Proteomics research with bioinformatics approach combined with the established two dimensional electrophoresis (2DE) platforms was studied by comparing yak with beef cattle muscle. Aiming to illustrate the causes and pathway of different meat qualities in yak and beef cattle, establish the optimal 2DE system and analyze protein bioinformatics pathways, different lysis buffer components, isoelectric focusing procedures and staining methods were studied by using longissimus dorsi muscle of yak. Proteomic profiling by 2DE and mass spectrometry identified 19 proteins that were differentially expressed in longissimus dorsi muscle of yak and beef cattle. Then the identified proteins were analyzed by gene ontology (GO) annotations and Kyoto encyclopedia of genes and genomes (KEGG) pathway. Results showed that the optimal protein extraction methods were lysis buffer component II, progressive fast boosting program and improved coomassie blue staining method. And protein spots in yak and beef cattle were 553 and 479, respectively. Totally 19 protein spots exhibiting a teo fold or more intensity difference in the meantime associated with 5% statistical significance (P<0.05) were considered differentially abundant. The differentially abundant proteins between yak and beef cattle could be divided into three main functional categories: metabolism proteins, structure proteins and stress proteins. The method of GO annotation provided three detailed and structured terms that included cellular component, molecular function and biological process. The differentially expressed proteins in yak and beef cattle muscle were concentrated in cellular processes, carbohydrate metabolism, genetic information processing and energy metabolism pathways by KEGG pathway analysis. In conclusion, the research result demonstrated the functions of identified proteins and provided a more detailed molecular view of the processes behind meat quality in yak and beef cattle.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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