邱硕,贾富国,韩燕龙,蒋龙伟,曾勇.基于分段加湿法的待发芽糙米臭氧水预处理工艺优化[J].农业机械学报,2016,47(12):271-278.
Qiu Shuo,Jia Fuguo,Han Yanlong,Jiang Longwei,Zeng Yong.Optimization of Ozonated Water Pretreatment for Brown Rice before Germination Based on Segmented Moisture Conditioning Method[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(12):271-278.
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基于分段加湿法的待发芽糙米臭氧水预处理工艺优化   [下载全文]
Optimization of Ozonated Water Pretreatment for Brown Rice before Germination Based on Segmented Moisture Conditioning Method   [Download Pdf][in English]
投稿时间:2016-05-12  
DOI:10.6041/j.issn.1000-1298.2016.12.033
中文关键词:  发芽糙米  臭氧水  灭菌率  发芽率  γ-氨基丁酸
基金项目:国家自然科学基金项目(51575098)、黑龙江省自然科学基金项目(E201322)和哈尔滨市应用技术与开发项目(2013DB2BG005)
作者单位
邱硕 东北农业大学 
贾富国 东北农业大学 
韩燕龙 东北农业大学 
蒋龙伟 东北农业大学 
曾勇 东北农业大学 
中文摘要:糙米吸湿发芽过程中微生物繁殖给发芽糙米带来安全隐患。为保障发芽糙米的安全性,研究基于分段加湿法的臭氧水灭菌预处理待发芽糙米工艺。以分段加湿后糙米为原料,研究糙米含水率、臭氧水初始质量浓度、臭氧水处理时间、臭氧水温度对灭菌率和发芽率的影响规律。采用二次正交旋转中心组合设计进行试验,建立了各因素对灭菌率和发芽率影响的数学模型。结果表明灭菌率、发芽率与各参数间回归方程极显著(P<0.01),优化参数组合为糙米含水率27.5%、臭氧水初始质量浓度4.7mg/L、臭氧水处理时间6.5min、臭氧水温度29.5℃,该条件下灭菌率和发芽率分别为(97.49±0.11)%和(91.89±0.26)%。与分段加湿后无灭菌处理相比,臭氧水预处理后发芽糙米菌落菌体浓度降低约5.20 lg CFU/g,发芽率和γ-氨基丁酸含量分别提高约0.49%和1.23mg/(100g)。研究证实优化后的预处理工艺既可有效灭菌又有利于糙米发芽。
Qiu Shuo  Jia Fuguo  Han Yanlong  Jiang Longwei  Zeng Yong
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:germinated brown rice  ozonated water  disinfection  germination rate  γ-aminobutyric acid
Abstract:To increase moisture of brown rice before germination, traditional soaking method provides a suitable environment for the growth of microorganisms, and segmented moisture conditioning method has no proper disinfection technique, which brings potential safety hazard to germinated brown rice. Therefore, an ozonated water treatment technique was proposed to enhance safety of brown rice before and after germination. The segmented moisture conditioned brown rice was selected as raw materials, with Design-Expert 8.0.6 software, a central composite rotatable orthogonal experimental design with four factors and five levels was employed to study effect of brown rice moisture content, initial mass concentration, treatment time and temperature of ozonated water on disinfection rate in aerobic plate count and germination rate of brown rice, and mathematical models for effect of each parameters on disinfection rate in aerobic plate count and germination rate were established. The mathematical models were extremely significant and parameters were optimized to increase disinfection rate in aerobic plate count above 97% and germination rate above 90%, and brown rice moisture content of 27.5%, initial mass concentration, treatment time and temperature of 4.7mg/L, 6.5min and 29.5℃ were decided to be optimal levels. Under this treatment condition, actual disinfection rate in aerobic plate count and germination rate were (97.49±0.11)% and (91.89±0.26)%. Compared with segmented moisture conditioning method without disinfection treatment, germinated brown rice produced with ozonated water treatment technique had significantly reduced amount of aerobic plate counts (P<0.01), and germination rate and γ-aminobutyric acid content were increased by 0.49% and 1.23mg/(100g). These verified that ozonated water pretreatment effectively disinfected aerobic plate count and increased germination rate of brown rice, which offered a basis for safety production of germinated brown rice.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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