Oxygation, which can impose aerated water to root zoon through Venturi tube and subsurface drip irrigation (SDI), has been showing yield improvement potentials and great application prospects. The impacts of climatic factors and soil temperature, oxygen concentration and moisture on soil respiration under oxygation and no aerated subsurface drip irrigation conditions were explored. Consequently, two treatments with three replicates were included. Plot experiments were conducted in a greenhouse during tomato growing season under these two different irrigation scenarios. The seasonal variation of soil respiration rate, soil temperature, soil oxygen concentration and soil moisture were described to investigate the relativity between soil respiration rate and these factors. Results showed that with oxygation and CK, soil temperature at the depth of 5cm (T5) had a high significant positive correlation with soil respiration rate (Rs), and the correlation coefficients were 0.615 and 0.564, respectively. Meanwhile, under the oxygation and CK conditions, T5 explained 46.6% and 32.4% variation of Rs, respectively. In addition, air humidity (RH) and soil oxygen concentration (Os) also affected the variation of Rs. RH explained 35.2% and 23.7% variation of Rs under the oxygation and CK conditions, respectively. And Os explained about 20% variation of Rs for the two treatments. The interaction of these factors affected 76.8% and 42.5% variation of Rs with oxygation and CK, respectively. Therefore, soil temperature was a controlling factor that constrained the soil respiration. Air humidity and soil oxygen concentration also were the important factors affecting the variation of soil respiration. Meanwhile, these factors had an interactive effect on variation of soil respiration rate, and the fitting result under oxygation was more effective. In addition, soil moisture was declined slightly with oxygation. The soil respiration rate and soil oxygen concentration under the oxygation conditions had a significant difference with CK, which were increased by 33.16% and 16.61% compared with CK, respectively. Thus, oxygation could improve soil environment effectively, and then decrease the limiting factors of soil respiration. As a result, under oxygation conditions, soil respiration was more sensitive to the interactions of soil temperature, relative humidity, soil moisture and soil oxygen concentration, and the fitness between these factors and soil respiration was better.