Reasonable partition of basic farmland construction is of great significance to arable land protection and food security. Based on farmland classification data and taking Ji’an county of Jiangxi province as the study area, this study selected arable land area ratio, fragmentation index, fractal dimension index and integrate index of connectivity for comprehensive evaluation of arable land spatial layout. Then, with the method of local Morans I spatial autocorrelation, the spatial structural characteristics and agglomerate regulation of arable land quality were explored by taking the arable land use quality index as the spatial variable. Finally, by spatial superposition combination, the spatial layout planning of basic farmland construction was given. The results showed that the arable land of the study area was divided into 2062 units which were arable land with 1km×1km grid. The arable land spatial layout was classified into three grades: first grade, second grade and third grade. The local spatial autocorrelation was divided into 4 types, including HH, LL, HL and LH. The basic farmland spatial layout was divided into priority delimited type, centralized regulation type and reserved development type, whose arable land ratio were 36.26%, 22.70% and 41.04%, respectively. The priority delimited type distributed in the central, including Litian, Meitang, Denglong, Guantian towns and eastern part of the study area. The centralized regulation type mainly distributed in northern study area, including Wanfu, Tongping towns and parts of southern study area. The reserved development type distributed in northern Ji’an county and eastern Ji’an county. With considering of arable land spatial layout and spatial agglomerate regulation of arable land quality, this study can provide reference for layout planning of the basic farmland construction.