刘方春,马海林,杜振宇,马丙尧,杨庆山,井大炜.根际促生细菌应用方式下金银花生长与根际环境特征[J].农业机械学报,2016,47(11):163-171.
Liu Fangchun,Ma Hailin,Du Zhenyu,Ma Bingyao,Yang Qingshan,Jing Dawei.Lonicera japonica Thunb. Growth and Its Rhizosphere Environment Characteristics with Application of Plant Growth promoting Rhizobacteria[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(11):163-171.
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根际促生细菌应用方式下金银花生长与根际环境特征   [下载全文]
Lonicera japonica Thunb. Growth and Its Rhizosphere Environment Characteristics with Application of Plant Growth promoting Rhizobacteria   [Download Pdf][in English]
投稿时间:2016-07-14  
DOI:10.6041/j.issn.1000-1298.2016.11.022
中文关键词:  金银花  植物根际促生细菌  交联聚丙烯酰胺  凝胶  根际
基金项目:国家自然科学基金项目(31570614)和山东省科技发展计划项目(2010GSF10621、2014GNC113006)
作者单位
刘方春 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心 
马海林 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心 
杜振宇 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心 
马丙尧 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心 
杨庆山 山东省林业科学研究院山东省森林植被生态修复工程技术研究中心 
井大炜 德州学院 
中文摘要:为提高植物根际促生细菌在干旱环境下的应用效果,以Bacillus cereus DZ1为供试菌株,探讨了清水稀释和豆芽汁营养液稀释处理下其在交联聚丙烯酰胺(CLP)中的存活特征,然后通过金银花造林实验,研究了接种B. subtilis DZ1(PGPR)、施用CLP(CLP)、接种B. subtilis DZ1并混施CLP(C-P)和CLP-PGPR凝胶(CPG)对植物生长及根际环境微生物特征的影响。结果表明,同清水稀释处理相比,豆芽汁营养液稀释处理CLP中的有效活菌数量显著提高19.45%~664.05%,CLP可以作为PGPR的吸附载体。同PGPR处理相比,CPG处理根系分泌物中的氨基酸总量、有机酸总量和总糖含量分别提高37.36%、30.04%和8.18%;直接接种B. cereus DZ1对根际土壤微生物量碳影响较小,但CPG处理显著提高了微生物量碳含量,并明显增强了土壤微生物呼吸作用,其微生物呼吸速率较PGPR、CLP、C-P和CK分别高出18.02%、9.93%、8.56%和18.91%;CPG处理还降低了代谢熵,与另外4个处理相比其下降幅度分别为12.74%、7.62%、8.02%和14.02%。无论B. cereus DZ1何种接种方式均对金银花的造林成活率影响不显著,但CPG处理显著增加了植物的干物质积累量,其叶片相对含水率较C-P处理显著增加10.34%,而相对电导率降低9.70%。可见,CLP-PGPR凝胶方式更有利于接种微生物的定殖存活,可改善金银花根际土壤的微生态环境,增强植物干旱耐受性的同时促进植物生长。
Liu Fangchun  Ma Hailin  Du Zhenyu  Ma Bingyao  Yang Qingshan  Jing Dawei
Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Shandong Academy of Forest,Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Shandong Academy of Forest,Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Shandong Academy of Forest,Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Shandong Academy of Forest,Shandong Engineering Research Center for Ecological Restoration of Forest Vegetation, Shandong Academy of Forest and Dezhou University
Key Words:Lonicera japonica Thunb.  plant growth promoting rhizobacteria  cross linked poly acrylamide  gel  rhizosphere
Abstract:Plant growth promoting rhizobacteria (PGPR) are beneficial native soil bacteria that colonize the rhizosphere or plant roots and regulate the functional properties of agricultural systems. One of the proposed mechanisms through which PGPR enhances plant growth is the production of plant growth regulators, especially cytokinin. However, little information is available regarding cytokinin producing PGPR inoculation on growth and water stress consistence of Lonicera japonica Thunb. seedlings. Therefore, a bacterial strain with high cytokinin production and known positive effects on plant growth was selected for use in present investigation. Cross linked polyacrylamide is a widely studied super absorbent polymer with segments of hydrophilic groups that can absorb and retain liquids, with the absorbed water it is difficult to remove even under pressure. In addition, because of the water absorbing properties of these macromolecules, cross linked polyacrylamide were used in a wide range of applications in agriculture and forestry. Firstly, an inoculation experiment was conducted to determine whether cross linked polyacrylamide could be used as carrier for PGPR by inoculating Bacillus cereus DZ1. And then, a Lonicera japonica Thunb. afforestation experiment, including five treatments, i.[KG-*3]e. PGPR (B. subtilis DZ1 inoculation alone), CLP (cross linked polyacrylamide was applied alone), C-P (B. subtilis inoculation with CLP), CPG (gel was made of PGPR and CLP), and CK (neither B. subtilis nor CLP was applied) was conducted. The experiment was to evaluate the effect of different ways of PGPR application on Lonicera japonica Thunb. growth and microbial characteristics in rhizosphere soils. Results indicated that compared with the diluted water treatment, the number of effective viable bacteria of bean sprout juice dilution treatment in cross linked polyacrylamide was significantly increased by 19.45%~664.05%, which meant that CLP can be used as an carrier for B. subtilis DZ1. The root exudates, namely amino acids, organic acids and total sugars in CPG treated Lonicera japonica Thunb. rhizosphere soils were significantly increased by 37.36%, 30.04% and 8.18% than those in PGPR treatment. Although B. subtilis DZ1 inoculation alone had no significant effect on microbial biomass carbon contents, the CPG treatment evidently increased microbial biomass carbon and significantly enhanced microbial respiration, showing 18.02%, 9.93%, 8.56% and 18.91% increases in microbial respiration rate over the treatments of PGPR, CLP, C-P and CK, respectively. At the same time, the metabolic quotient was reduced by the CPG treatment, demonstrating 12.74%, 7.62%, 8.02% and 14.02% decreases, respectively. Besides, the CPG treatment achieved the highest Simpson index, however the lowest Shannon and McIntosh index among all the treatments. CPG treatment had no significant effect on afforestation survival rate, it was beneficial to the shoot and root dry matter accumulation of Lonicera japonica Thunb. Compared with C-P treatment, CPG treatment increased the relative water content of Lonicera japonica Thunb. leaves by 10.34%, whereas decreased the relative electric conductance by 9.70%. As a result, the CLP-PGPR gel can alleviate the drought stress and interfere with the suppression of plant growth through regulating the micro ecological environment of Lonicera japonica Thunb. rhizosphere soil, showing a real potential to perform as a drought stress inhibitor in arid environments.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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