郭全友,修艳辉,姜朝军,张淑平,宋炜,田冬梅.环境因子对大黄鱼腐败希瓦氏菌生长影响的计数法分析[J].农业机械学报,2016,47(10):250-257,317.
Guo Quanyou,Xiu Yanhui,Jiang Chaojun,Zhang Shuping,Song Wei,Tian Dongmei.Effect of Environmental Factors on Growth of Shewanella putrefaciens Based on Different Counting Methods[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(10):250-257,317.
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环境因子对大黄鱼腐败希瓦氏菌生长影响的计数法分析   [下载全文]
Effect of Environmental Factors on Growth of Shewanella putrefaciens Based on Different Counting Methods   [Download Pdf][in English]
投稿时间:2016-03-24  
DOI:10.6041/j.issn.1000-1298.2016.10.031
中文关键词:  大黄鱼  腐败希瓦氏菌  平板计数法  浊度  环境因子  生长动力学参数
基金项目:国家自然科学基金项目(31371867)、上海市自然科学基金项目(16ZR1444900)、中国水产科学研究院东海水产研究所基本科研业务费项目(2014G02)、创新训练项目(201510252095)和南昌大学食品科学与技术国家重点实验室开放基金项目(SKLF—KF—201607)
作者单位
郭全友 中国水产科学研究院 
修艳辉 中国水产科学研究院
上海海洋大学 
姜朝军 中国水产科学研究院 
张淑平 上海理工大学 
宋炜 中国水产科学研究院 
田冬梅 上海理工大学 
中文摘要:以分离于腐败大黄鱼中的腐败希瓦氏菌为对象,采用平板计数法和浊度法分析不同pH值、NaCl浓度和温度下的生长情况,运用修正的Gompertz方程拟合生长曲线,用决定系数、准确度、精确度、残差平方和及平方根误差评价拟合优度,比较分析2种计数法下环境因子对生长界限和动力学参数的影响。结果表明,在3~8℃,pH值6.0及NaCl质量分数3.5%时不生长;2种计数法下生长动力学参数最大比生长速率(μmax)和代时差异不显著(p>0.05),而迟滞期(λ)和最大细胞密度差异显著(p<0.05);环境因子对生长动力学参数有显著影响,温度和NaCl浓度对代时影响较大,温度越高或NaCl浓度越低,代时越短;在3~25℃,温度越高,μmax越大,增速为(0.0143±0.0032)h-1/℃,温度越高,λ越小,平板计数法下其降速约1.5h/℃,而在8~15℃,浊度法下λ降速约2.5h/℃;平板计数法下μmax的变化受NaCl浓度及pH值影响较大,λ变化无明显规律,而浊度法下μmax的变化受NaCl浓度和pH值影响不明显,λ变化较明显。因此2种计数法均可对腐败希瓦氏菌生长/非生长作出有效判断,环境因子强度较大时浊度法相比平板技数法迟滞期与最大细胞密度误差较大。
Guo Quanyou  Xiu Yanhui  Jiang Chaojun  Zhang Shuping  Song Wei  Tian Dongmei
Chinese Academy of Fishery Sciences,Chinese Academy of Fishery Sciences;Shanghai Ocean University,Chinese Academy of Fishery Sciences,Shanghai Ocean University,Chinese Academy of Fishery Sciences and Shanghai Ocean University
Key Words:large yellow croaker  Shewanella putrefaciens  viable count method  optical density method  environmental factors  growth kinetic parameters
Abstract:The Shewanella separated from corruption large yellow croaker was used as research object. The growth data of Shewanella putrefaciens under different pH values, NaCl concentrations and temperatures was obtained by using viable count and optical density methods, and the Gompertz model was built. Effect of environmental factors on growth kinetic parameters was compared and analyzed. Results showed that Shewanella putrefaciens could not grow at pH value of 6.0 and NaCl concentration of 3.5% during 3~8℃. The differences of maximum specific growth rate (μmax) and generation time were not significant (p>0.05), but the differences of lag phase (λ) and the most probable density (M) were significant (p<0.05). Environmental factors had significant effect on the growth kinetic parameters.High temperature or low NaCl concentration resulted in short generation time. When temperature was 3~25℃, high temperature resulted in large μmax. The increase rate of μmax was (0.0143±0.0032)h-1/℃.When temperature was high, λ would be short. The λ was decreased at a rate of 1.5h/℃ by using viable count method, while during 8~15℃, it was decreased approximately at a speed of 2.5h/℃ by optical density method. The μmax was influenced significantly by NaCl concentration and pH value, while lag time showed no significant changes by viable count method. Using optical density method, changes of μmaxwere affected unsignificantly by NaCl concentration and pH value, while changes of λ were apparent. Thus, both of the two counting methods could judge the growth/non growth state of Shewanella, and the estimation errors of λ and M were increased by harsh environments when using optical density methods.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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