江慎华,万严,杨琼玉,黄泽勇,张化浩,马海乐.丁香有效部位对低密度脂蛋白弱氧化修饰的抑制效果[J].农业机械学报,2016,47(6):242-249.
Jiang Shenhua,Wan Yan,Yang Qiongyu,Huang Zeyong,Zhang Huahao,Ma Haile.Antioxidant Inhibition Effect of Effective Fraction of Clove on Minimally Modified Low Density Lipoprotein[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(6):242-249.
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丁香有效部位对低密度脂蛋白弱氧化修饰的抑制效果   [下载全文]
Antioxidant Inhibition Effect of Effective Fraction of Clove on Minimally Modified Low Density Lipoprotein   [Download Pdf][in English]
投稿时间:2016-02-26  
DOI:10.6041/j.issn.1000-1298.2016.06.032
中文关键词:  丁香  低密度脂蛋白  弱氧化  氧化修饰
基金项目:国家自然科学基金项目(31360371、31560308)、江西省自然科学基金项目(20132BAB204030)、江西省科技支撑计划项目(20151BBF60026)、江西省卫生厅科研计划项目(2013A017)、江苏省农产品物理加工重点实验室开放课题项目(JAPP2010—5)、江西省天然产物与功能食品重点实验室开放基金项目、九江市科技支撑计划项目(201438)和九江学院教学改革研究课题(2015—04)
作者单位
江慎华 九江学院
江苏大学 
万严 九江学院 
杨琼玉 九江学院 
黄泽勇 九江学院 
张化浩 九江学院 
马海乐 九江学院
江苏大学 
中文摘要:低密度脂蛋白(LDL)弱氧化修饰是形成动脉粥样硬化(AS)的关键因素。为了研究丁香有效部位(EFC)对LDL弱氧化修饰的抑制效果,采用Fe2+体外诱导LDL弱氧化,通过测定脂质过氧化产物共轭二烯(CD)、硫代巴比妥酸反应物(TBARS)的产生、LDL紫外可见光谱及载脂蛋白B—100(apoB—100)中赖氨酸(Lys)游离氨基、色氨酸(Trp)活性的衰减程度反映抑制效果。结果表明,在LDL弱氧化过程中,对脂质过氧化保护方面, EFC(2.5μg/mL)能有效延缓增殖阶段CD产生,其延缓效果强于0.5μg/mL阳性对照。EFC(50μg/mL)能延缓LDL降解阶段TBARS生成,延缓效果强于10μg/mL BHT;相应质量浓度(1.25μg/mL、1.5μg/mL)的EFC能减缓LDL因为氧化而改变的紫外可见光谱。对蛋白(apoB—100)氧化保护方面,EFC(5μg/mL)能显著抑制Lys中游离氨基活性衰减(P<0.05),其抑制效果与1.25μg/mL BHT无显著性差异;EFC(1μg/mL)能保护apoB—100中Trp免受破坏。结果表明EFC能有效抑制LDL弱氧化,为后续相关功能食品研发提供了参考。
Jiang Shenhua  Wan Yan  Yang Qiongyu  Huang Zeyong  Zhang Huahao  Ma Haile
Jiujiang University;Jiangsu University,Jiujiang University,Jiujiang University,Jiujiang University,Jiujiang University and Jiujiang University;Jiangsu University
Key Words:clove  low density lipoprotein  antioxidation  oxidative modification
Abstract:Minimally modified low density lipoprotein (mm-LDL) plays an important role in the progression of atherosclerosis. In order to further study the inhibition effect of effective fraction of clove (EFC) on mm-LDL, antioxidation was induced by ferrous sulfate in vitro and inhibition effect was examined by the following methods: determination of conjugated dienes (CD), thiobarbituric acid reactive substances (TBARS), UV-visible spectra, LDL-tryptophan fluorescence, free amino groups on lysine. The results indicated that EFC (2.5μg/mL) effectively delayed the CD to be generated, and this delay effect of EFC was better than that of positive control-BHT (0.5μg/mL) in the course of oxidation propagation. EFC (50μg/mL)also put off the generation of TBARS during the decomposition of LDL, this delay effection of EFC was better than that of BHT(10μg/mL). Likewise, the UV-visible spectra of LDL during the course of oxidation could be protected from being changed by EFC with certain concentrations (1.25μg/mL, 1.5μg/mL). Meanwhile, EFC (5μg/mL) could significantly inhibit the decay of free amino groups on lysine in terms of protection on protein (apoB—100) oxidation of LDL , and this fraction (1μg/mL) may effectively protect LDL-tryptophan from destruction (P<0.05), furthermore, this distinction of inhibition effect was not significant with BHT(1.25μg/mL). In conclusion, these results proved that EFC had a significantly antioxidant activity on mild oxidation of LDL,which provides reference for further research and development of functional food.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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