张哲,赵静,田津津,王怀文,王飒飒,张平.冷冻-复温过程中葡萄细胞结晶变化研究[J].农业机械学报,2016,47(5):211-217.
Zhang Zhe,Zhao Jing,Tian Jinjin,Wang Huaiwen,Wang Sasa,Zhang Ping.Research on Crystallization Change of Grape Cells during Freezing-thawing Process[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(5):211-217.
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冷冻-复温过程中葡萄细胞结晶变化研究   [下载全文]
Research on Crystallization Change of Grape Cells during Freezing-thawing Process   [Download Pdf][in English]
投稿时间:2016-02-25  
DOI:10.6041/j.issn.1000-1298.2016.05.029
中文关键词:  葡萄细胞  冷冻复温  结晶变化  低温显微镜
基金项目:国家自然科学基金项目(11572223)和天津市自然科学基金重点项目(14JCZDJC34600、15JCZDJC34200)
作者单位
张哲 天津商业大学 
赵静 天津商业大学 
田津津 天津商业大学 
王怀文 天津商业大学 
王飒飒 天津商业大学 
张平 国家农产品保鲜工程技术研究中心天津市农产品采后生理与贮藏保鲜重点实验室 
中文摘要:利用低温显微镜系统对葡萄进行了冷冻-复温实验,研究了葡萄细胞在冷冻-复温过程的结晶变化。通过分析冷冻-复温过程的葡萄细胞显微图像、细胞体积、内压、渗透率的变化,发现:葡萄细胞的结冰温度随冷冻速率的增加而降低;葡萄细胞的体积变化总趋势是随温度的降低而减小,但由于细胞内自由水体积的变化,在-5~0℃细胞体积会有所增大。在14℃/min、50℃/min冷冻速率下细胞体积分别减小了54.5%,26.3%。冷冻速率越大,细胞体积变化越小;冷冻过程中葡萄细胞的渗透系数随温度的降低而降低,随冷冻速率的降低而降低。在葡萄细胞的冷冻-复温过程中,提高复温温度能有效减少细胞的破损。
Zhang Zhe  Zhao Jing  Tian Jinjin  Wang Huaiwen  Wang Sasa  Zhang Ping
Tianjin University of Commerce,Tianjin University of Commerce,Tianjin University of Commerce,Tianjin University of Commerce,Tianjin University of Commerce and Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, National Engineering and Technology Research Center for Preservation of Agricultural Products
Key Words:grape cell  freezing thawing  crystalline change  cryomicroscope
Abstract:The freezing thawing phenomenons of fruits and vegetables are inevitable during the refrigerated transport process. The quality of fruits and vegetables will also be changed. Grapes as test objects are used to test the quality change during the freezing thawing process. The cryomicroscope system (including CCD camera, BCS 196 cryostage, the liquid nitrogen tank, the crystage temperature control system) was used to test the grape sample during the freezing thawing process. The temperature range of cryomicroscope system is -196℃ to 125℃. The crystallization change of grape cells has been researched. The freezing thawing rate is 2,6,8,11,14,20,50, 90℃/min. Micrograph, volume, internal pressure and permeability coefficient of grape cells were analyzed during the freezing thawing process. The result shows that the formation process of crystallization in the intercellular space depends on the freezing rate. Grape cell freezing temperatures will decrease with increasing freezing rate and the general trend of change in volume of grape cells will decrease with the decreasing of temperature. However, grape cells volume will increase at -5~0℃ due to the volume changes of intracellular free water. Grape cells volumes were reduced by 54.5%, 26.3% respectively at 14℃/min and 50℃/min freezing rates. The larger the freezing rate is, the smaller the cell volume changes to be. During the freezing process, the permeability coefficient of grape cells will decrease with the decreasing temperature and freezing rate. Increasing thawing temperature can effectively reduce the damage of the grape cells during freezing thawing process. This research will optimize the temperature gradient of fruit and vegetable during cold storage.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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