郭建斌,郭亚琳,赵倩,吴树彪,董仁杰.真菌固态载体预处理对不灭菌玉米秸秆降解的影响[J].农业机械学报,2016,47(5):197-202.
Guo Jianbin,Guo Yalin,Zhao Qian,Wu Shubiao,Dong Renjie.Effect of Precolonized Fungi Carrier on Lignin Degradation of Corn Straw under Unsterilized Condition[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(5):197-202.
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真菌固态载体预处理对不灭菌玉米秸秆降解的影响   [下载全文]
Effect of Precolonized Fungi Carrier on Lignin Degradation of Corn Straw under Unsterilized Condition   [Download Pdf][in English]
投稿时间:2015-11-27  
DOI:10.6041/j.issn.1000-1298.2016.05.027
中文关键词:  玉米秸秆  木质素  真菌预处理  载体  灭菌
基金项目:中央高校基本科研业务费专项资金项目(15055171)、“十二五”国家科技支撑计划项目(2015BAD21B04)、北京市科技计划项目(D141100001214001、Z151100001115010)和中欧国际合作项目(SQ2013Z0A000017)
作者单位
郭建斌 中国农业大学 
郭亚琳 中国农业大学 
赵倩 中国农业大学 
吴树彪 中国农业大学 
董仁杰 中国农业大学 
中文摘要:采用云芝变色栓菌(Trametes versicolor)、色精木霉菌(Trichoderma chromospermum)、深绿木霉菌(Trichoderma atroviride)和云芝变色栓菌分别与色精木霉菌、深绿木霉菌的混合菌对干黄玉米秸秆进行真菌预处理。结果显示,真菌预处理不灭菌秸秆30d后,木质纤维素的降解率几乎可以忽略,在不灭菌的环境中,接入的真菌对秸秆本身附着的微生物群落不能形成竞争优势,造成较低的木质素降解率。而云芝变色栓菌、色精木霉菌、深绿木霉菌以及云芝变色栓菌分别与色精木霉菌、深绿木霉菌的混合菌对灭菌秸秆预处理30d后,木质素的降解率分别为34.0%、38.1%、38.1%、39.1%及 40.3%。为降低秸秆的灭菌成本,将真菌预处理30d后的灭菌秸秆作为真菌固态载体与不灭菌秸秆按照1∶9、1∶4及 1∶1的干质量比混合培养30d,结果显示,采用云芝变色栓菌分别与色精木霉菌和深绿木霉菌的混合菌固态载体预处理不灭菌秸秆,木质素降解率可达到30%~40%。因而,真菌固态载体可以较好地适应非灭菌环境并对秸秆中的木质素进行有效降解,是一种有效降低过程成本的预处理手段。
Guo Jianbin  Guo Yalin  Zhao Qian  Wu Shubiao  Dong Renjie
China Agricultural University,China Agricultural University,China Agricultural University,China Agricultural University and China Agricultural University
Key Words:corn straw  lignin  fungal pretreatment  carrier  sterilization
Abstract:Delignification is crucial in the energy utilization process of lignocellulose materials. In this study, efficiency of biological pretreatment of corn straw using different carriers of pre colonized fungis, including Trametes versicolor, Trichoderma chromospermum, Trichoderma atroviride and combined fungi (T. versicolor + T. chromospermum, T. versicolor + T. atroviride) was investigated under unsterilized condition. Results showed that nearly negligible lignin degraded in the process of direct fungal pretreatment of unsterilized corn straw within 30d. It seems that inoculum of fungi cannot compete to the indigenous microorganisms of unsterilized corn straw, resulting in low lignin degradation rate. However, the degradation rates of 34.0%, 38.1%, 38.1%, 39.1% and 40.3% lignin in the sterilized corn straw were observed after 30d of cultivation by T. versicolor, T. chromospermum, T. atroviride, T. versicolor + T. chromospermum and T. versicolor + T. atroviride, respectively. To decrease the cost of sterilization, corn straw was pretreated by T. versicolor + T. atroviride and T. versicolor + T. atroviride with 1∶9, 1∶4 and 1∶1 of the ratio of pre colonized carrier and substrate for 30d. During T. versicolor + T. chromospermum and T. versicolor + T. atroviride, pretreatment with an inoculum/substrate ratio of 1∶9, 1∶4 and 1∶1, the degradation rate of lignin were 30% ~ 40%. Therefore, pre colonized carrier inoculum is an effective pretreatment method to decrease cost due to pre colonized carrier can adjust the unsterilized condition and degrade lignin effectively. 

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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