To explore the visual acuity reaction effect of Locusta migratoria responding to LED light signal, clear the photosensitive response factor and the biological light inducing parameters of Locusta migratoria phototaxis regulated by light, the spectral representation of Locusta migratoria visual reaction stimulated by LED light source was investigated with AvaSpec fiber-optic spectrometer system. On this basis, the degree of Locusta migratoria phototactic response and reaction regulated by spectral light was certificated comparatively through utilizing the investigated device of Locusta migratoria phototactic response to spectral illumination. The results showed that the visual reaction was represented to 380nm, 402nm, 540nm and 602nm glimmer characteristics when Locusta migratoria visual system accepted to absorb 365nm, 400nm, 520nm, and 610nm light photons, and spectral illumination was enhanced, the visual spectral transmission peak amplitude was increased to take on the reaction characteristics of time-varying effect, which originated from the difference of photophysicochemical reactions and light-sensing deconstruction effect of the visual system absorbing different spectral photons, and the visual sensitivity degree of responding to different spectral light stimulation was manifested by visual spectrum peak misregistration and visual spectrum emission amplitude. Moreover, timevarying intensity of Locusta migratoria visual photosensitivity regulation to violet light was the optimal, the following was orange light, and green light was the worst, the visual photosensitivity regulation capacity for orange light was the optimal, the following was green light, and violet light was the worst. Simultaneously, the superior photosensitive aggregating speed for UV light, the better photosensitive aggregating degree for violet light and the better phototactic effect for orange light showed that the phototactic photosensitivity activity, phototactic degree and the phototactic effect were positively correlated with visual stimulation intensity, tim-varying intensity of visual photosensitivity regulation and visual photosensitivity regulation capacity, respectively, and the photosensitive time effectiveness of the visual reaction intensity affected the behavioral selection effect and visual photosensitivity activity effect. Then, based on the stronger stimulating effect of UV light, the better time effectiveness sensitivity of orange light and the stronger photosensitive regulation intensity of violet light, Locusta migratoria phototactic effect can be improved by stimulating time regulation with the combination of UV, violet and orange light. Research results provided theoretical support for the study of trapping mechanical equipment of Locusta migratoria induced by light.