董庆利,石育娇,刘箐,刘阳泰,张春江,刘弘.酸化和渗透压不同处理顺序对肠炎沙门菌失活的影响[J].农业机械学报,2016,47(4):201-208.
Dong Qingli,Shi Yujiao,Liu Qing,Liu Yangtai,Zhang Chunjiang,Liu Hong.Effect of Processing Sequence of Acid and Osmotic Pressure on Salmonella enteritis Inactivation[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(4):201-208.
摘要点击次数: 945
全文下载次数: 795
酸化和渗透压不同处理顺序对肠炎沙门菌失活的影响   [下载全文]
Effect of Processing Sequence of Acid and Osmotic Pressure on Salmonella enteritis Inactivation   [Download Pdf][in English]
投稿时间:2015-09-14  
DOI:10.6041/j.issn.1000-1298.2016.04.027
中文关键词:  肠炎沙门菌  失活  酸化  渗透压  数学模型
基金项目:国家自然科学基金项目(31271896、31371776)、上海市科委重点支撑项目(13430502400)和上海市科委长三角科技联合攻关领域项目(15395810900)
作者单位
董庆利 上海理工大学 
石育娇 上海理工大学 
刘箐 上海理工大学 
刘阳泰 上海理工大学 
张春江 中国农业科学院 
刘弘 上海市疾病预防控制中心 
中文摘要:为探讨酸化和渗透压不同处理顺序对肠炎沙门菌失活的影响,运用统计学模型模拟了最优处理条件下,肠炎沙门菌在不同初始接菌量下的失活过程。实验结果表明:在25℃和10h内,先15%NaCl再pH值4.5处理使肠炎沙门菌失活的效果大于相反顺序的处理、同时处理或者单独处理的效果。模拟结果表明:在最优处理条件下的各个阶段中,随着初始接菌量的增加,肠炎沙门菌分别达到0.9864和0.9939失活率时对应的失活时间的变异性逐渐降低。对于大量群体细菌,用确定性失活动力学一级模型拟合的D值是单一的定值;而对少量群体细菌,由于个体细胞本身的变异性,D值表现出较高的变异性,并且可用概率分布的形式表示。酸化和渗透压不同处理顺序的研究能为食品工业生产控制致病菌提供借鉴,且应用的统计学建模方法有助于从个体细胞的角度理解群体失活过程,提高风险评估的准确性。
Dong Qingli  Shi Yujiao  Liu Qing  Liu Yangtai  Zhang Chunjiang  Liu Hong
University of Shanghai for Science and Technology,University of Shanghai for Science and Technology,University of Shanghai for Science and Technology,University of Shanghai for Science and Technology,Chinese Academy of Agricultural Sciences and Shanghai Municipal Center for Disease Control & Prevention
Key Words:Salmonella enteritis  inactivation  acid  osmotic pressure  mathematical model
Abstract:To study the influence of different processing sequences of acid and osmotic pressure on the inactivation of Salmonella enteritis, a statistical model was used to simulate the inactivation of Salmonella enteritis with different initial inoculations under the optimal processing conditions. The experimental results demonstrated that the sequence of 15% NaCl stress applied firstly and then pH value of 4.5 stress treatment made the number of Salmonella enteritis reduce faster than the reverse sequence or single stress or double stresses used simultaneously at 25℃ in 10h. Moreover, the simulation results indicated that the variability of inactivation time was reduced gradually with the increase of initial inoculation when the inactivation rate of Salmonella enteritis reached 0.9864 and 0.9939, respectively, at all stages of the optimal processing conditions. In addition, the D value by the deterministic inactivation model was a single constant value for a large number of bacteria. However, the fitted D value showed high variability for a small group of bacteria due to the variability of the individual cells, and the D value could be described by the probability distribution. Consequently, the research of different processing sequences of acid and osmotic stress could provide reference of controlling pathogens for food industry production, and the applied statistical modeling method would help to understand the inactivation from the perspective of individual cells as well as to improve the accuracy of risk assessment.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

   下载PDF阅读器