陈振家,施小迪,杜昱蒙,姚美伊,郭顺堂.不同杀菌处理方式对SPI冻藏过程中理化特性的影响[J].农业机械学报,2016,47(2):251-257.
Chen Zhenjia,Shi Xiaodi,Du Yumeng,Yao Meiyi,Guo Shuntang.Effects of Different Sterilization Conditions on Physicochemical Properties of SPI during Frozen Storage[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(2):251-257.
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不同杀菌处理方式对SPI冻藏过程中理化特性的影响   [下载全文]
Effects of Different Sterilization Conditions on Physicochemical Properties of SPI during Frozen Storage   [Download Pdf][in English]
投稿时间:2015-09-18  
DOI:10.6041/j.issn.1000-1298.2016.02.033
中文关键词:  大豆分离蛋白  杀菌  冻藏  理化特性
基金项目:“十二五”国家科技支撑计划项目(2012BAD34B04-2)和国家自然科学基金项目(31471582)
作者单位
陈振家 中国农业大学
山西农业大学 
施小迪 中国农业大学 
杜昱蒙 中国农业大学 
姚美伊 中国农业大学 
郭顺堂 中国农业大学 
中文摘要:为了研究不同杀菌条件对SPI理化特性的影响及相应SPI溶液冷冻条件下的物化性质变化,通过65℃、90℃和UHT不同杀菌处理所得SPI(分别记为65SPI、90SPI和USPI)制备相应蛋白溶液,经-18℃冻藏后,对其理化特性进行分析研究。试验结果表明:3种杀菌处理均造成了SPI一定程度的变性,与冻前未处理SPI相比,90SPI和65SPI溶液的可溶蛋白含量和表面巯基显著降低,粘度、粒径大幅增加,USPI则未有明显变化。此外,90SPI和USPI的疏水基团暴露程度较高,非还原电泳显示其AB亚基发生解离;不同杀菌处理SPI其溶液在冻藏期间内,随冻藏时间延长,蛋白溶解度、粘度、表面巯基和总巯基数量呈下降趋势,粒径和疏水基团暴露程度增加。冻藏后蛋白电泳图谱显示,65SPI和90SPI通过二硫键形成了分子量较大的可溶性聚集体。
Chen Zhenjia  Shi Xiaodi  Du Yumeng  Yao Meiyi  Guo Shuntang
China Agricultural University;Shanxi Agricultural University,China Agricultural University,China Agricultural University,China Agricultural University and China Agricultural University
Key Words:soybean protein isolate  sterilization  frozen storage  physicochemical properties
Abstract:The effects of different sterilization conditions on the physicochemical properties of soybean protein isolate (SPI) during frozen storage were studied to gain scientific knowledge and provide basis for supplying soy foods with good quality. SPI was prepared after sterilized at 65℃, 90℃ and UHT (denoted as 65SPI, 90SPI and USPI), respectively. The changes of physicochemical properties of protein dispersions (2%, pH value 7.0) subjected to frozen storage at -18℃ were investigated. The results showed that three types of sterilization all resulted in heat denaturation of proteins. Compared with control SPI, the soluble proteins content and free SH groups of 90SPI and 65SPI were dropped sharply while the viscosity and particle size were increased greatly. However, there were no marked changes of USPI. In addition, hydrophobic domains of 90SPI and USPI were exposed to a higher extent and their AB subunits were in dissociation state according to non-reducing SDS-PAGE. SPI with different sterilization treatments underwent different chemical and physical changes during frozen storage. The solubility, viscosity, free and total SH groups of protein trended down gradually with the increase of frozen time, while particle size and exposed extent of hydrophobic domains were increased during frozen storage. Some soluble aggregations associated with disulfide bonds were observed in the SDS-PAGE electrophoresis of 65SPI and 90SPI solutions after frozen storage.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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