蔡瑞,袁亚宏,王周利,郭春锋,刘斌,岳田利.ε-聚赖氨酸对脂环酸芽孢杆菌生长与产物代谢的影响[J].农业机械学报,2015,46(10):285-289,327.
Cai Rui,Yuan Yahong,Wang Zhouli,Guo Chunfeng,Liu Bin,Yue Tianli.Effect of ε-polylysine on Growth and Guaiacol Production of Alicyclobacillus spp.Production of Alicyclobacillus spp.[J].Transactions of the Chinese Society for Agricultural Machinery,2015,46(10):285-289,327.
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ε-聚赖氨酸对脂环酸芽孢杆菌生长与产物代谢的影响   [下载全文]
Effect of ε-polylysine on Growth and Guaiacol Production of Alicyclobacillus spp.Production of Alicyclobacillus spp.   [Download Pdf][in English]
投稿时间:2015-07-16  
DOI:10.6041/j.issn.1000-1298.2015.10.038
中文关键词:  脂环酸芽孢杆菌  ε-聚赖氨酸  抑菌  愈创木酚
基金项目:陕西省社会发展科技攻关资助项目(2015SF278)、港澳台科技合作专项资助项目(K304021501)、陕西省科学技术研究发展计划资助项目(2014K1315)和西北农林科技大学博士科研启动基金资助项目(Z109021503)
作者单位
蔡瑞 西北农林科技大学 
袁亚宏 西北农林科技大学 
王周利 西北农林科技大学 
郭春锋 西北农林科技大学 
刘斌 西北农林科技大学 
岳田利 西北农林科技大学 
中文摘要:研究了ε-聚赖氨酸对脂环酸芽孢杆菌属生长及产愈创木酚的影响。以A. acidoterrestris、A. herbarius和A. acidiphilus为研究对象,采用双倍稀释法测定最小抑菌浓度;通过菌落计数和高相液相色谱法检测愈创木酚的含量,评价ε-聚赖氨酸对模拟苹果汁体系中脂环酸芽孢杆菌生长代谢的影响。结果表明:ε-聚赖氨酸能够有效抑制脂环酸芽孢杆菌营养细胞和芽孢的生长,最小抑菌质量浓度为1.25~5 mg/L。当模拟苹果汁中ε-聚赖氨酸的添加量为最小抑菌浓度时,脂环酸芽孢杆菌的菌落数呈下降趋势,减少量为1~2个数量级;愈创木酚的生成量显著降低,质量浓度范围为1.7~4.74 mg/L。当ε-聚赖氨酸添加量增加至最小抑菌浓度的4倍时,菌落数由(5~6)×106个/mL降低至4.6×102个/mL以下,同时,可以完全抑制愈创木酚的产生。因此,ε-聚赖氨酸可以有效控制果汁中脂环酸芽孢杆菌属细菌腐败。
Cai Rui  Yuan Yahong  Wang Zhouli  Guo Chunfeng  Liu Bin  Yue Tianli
Northwest A&F University,Northwest A&F University,Northwest A&F University,Northwest A&F University,Northwest A&F University and Northwest A&F University
Key Words:Alicyclobacillus spp.  ε-polylysine  Growth inhibition  Guaiacol
Abstract:Alicyclobacillus spp. has recently received much attention due to its implication in the spoilage of pasteurized fruit juices, which was characterized by the production of a smoky, medicinal and antiseptic off-odor attributed to guaiacol. The species those are known to produce guaiacol are A. acidoterrestris, A. acidiphilus and A. herbarius. The effect of ε-polylysine on growth and guaicol production of these three species of Alicyclobacillus spp. was evaluated. Minimal inhibitory concentration (MIC) of ε-polylysine was determined in AAM medium by using broth dilution method. The sensitivity of Alicyclobacillus spp. to ε-polylysine was species-dependent, and MIC values ranged from 1.25 mg/mL to 5 mg/mL against vegetative cells and from 2.5 mg/mL to 5 mg/mL against the spores. Then, various concentrations of ε-polylysine were added to the model apple juice. When the dose of ε-polylysine increased to 4 MIC, Alicyclobacillus counts were reduced below 2.66 log colony forming unit (CFU)/mL, and no guaiacol was detected after 7 d incubation. The results can be very useful for better control of Alicyclobacillus-related spoilage in the fruit juice and beverage industry.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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