董庆利,王海梅,Pradeep K Malakar,刘阳泰.不同介质间采用矩阵量化气单胞菌的交叉污染[J].农业机械学报,2015,46(4):219-225,272.
Dong Qingli,Wang Haimei,Pradeep K Malakar,Liu Yangtai.Applying Matrix to Quantify Cross-contamination of Aeromonas spp. among Different Medium[J].Transactions of the Chinese Society for Agricultural Machinery,2015,46(4):219-225,272.
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不同介质间采用矩阵量化气单胞菌的交叉污染   [下载全文]
Applying Matrix to Quantify Cross-contamination of Aeromonas spp. among Different Medium   [Download Pdf][in English]
投稿时间:2015-01-13  
DOI:10.6041/j.issn.1000-1298.2015.04.032
中文关键词:  气单胞菌  交叉污染  矩阵  介质
基金项目:国家自然科学基金资助项目(31271896)和上海市自然科学基金资助项目(12ZR1420500)
作者单位
董庆利 上海理工大学 
王海梅 上海理工大学 
Pradeep K Malakar 食品研究所肠道健康与食品安全组 
刘阳泰 上海理工大学 
中文摘要:为定量描述气单胞菌在不同介质间的转移及变化过程,基于单个细菌在每次转移过程中只能被转移到一个介质表面的假设,采用矩阵方式将气单胞菌的转移路径及被转移的可能性集中于一个二维的数据表格之中,并对不同场景下气单胞菌在不同介质表面的交叉污染水平进行分析。结果表明,采用矩阵方式不仅可用于探究交叉污染对细菌受体表面带菌量的变化,同时可定量分析细菌供体表面带菌量的变化,如在分割冷却猪肉前、后,猪肉中气单胞菌的带菌量平均值分别为3.02 lg CFU/g、1.82 lg CFU/g。对不同场景下各介质带菌量的量化结果证实:分割冷却猪肉后,若及时对被污染的案板、刀具等介质进行清洗或更换,可有效降低交叉污染水平并可预防二次交叉污染的发生。
Dong Qingli  Wang Haimei  Pradeep K Malakar  Liu Yangtai
University of Shanghai for Science and Technology,University of Shanghai for Science and Technology,Gut Health and Food Safety, Institute of Food Research and University of Shanghai for Science and Technology
Key Words:Aeromonas spp.  Cross-contamination  Matrix  Medium
Abstract:The transfer approach and transfer probability of Aeromonas spp. under various food-handling scenarios were centralized in a two-dimensional data table by applying matrix method, which aimed at quantifying cross-contamination of Aeromonas spp. among different food-contact surfaces. Results showed that the number of Aeromonas spp. on donor and recipient surface could be calculated simultaneously, for instance, the average values of Aeromonas spp. in chilled pork before and after cross-contamination were 3.02 lg CFU/g and 1.82 lg CFU/g, respectively. Results also confirmed that proper conducts after splitting pork including cleaning contaminated boards, knives, hands and/or just changing them into clean ones before splitting Brassica chinensis could effectively reduce cross-contamination level. The study of microbial cross-contamination could be helpful for setting good hygienic practice guidelines for consumers, and it could provide a theoretical basis for assessing the potential risk of cross-contamination.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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