付为国,王凡坤,李萍萍,汤涓涓.铅对小白菜根际微生物群落与其生理功能的影响[J].农业机械学报,2014,45(12):203-208.
Fu Weiguo,Wang Fankun,Li Pingping,Tang Juanjuan.Effects of Lead on Microbial Communities in Rhizosphere and Physiological Functions of Brassica chinensis L.[J].Transactions of the Chinese Society for Agricultural Machinery,2014,45(12):203-208.
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铅对小白菜根际微生物群落与其生理功能的影响   [下载全文]
Effects of Lead on Microbial Communities in Rhizosphere and Physiological Functions of Brassica chinensis L.   [Download Pdf][in English]
投稿时间:2014-06-25  
DOI:10.6041/j.issn.1000-1298.2014.12.030
中文关键词:  小白菜  微生物  铅胁迫  硝化  反硝化  呼吸速率
基金项目:“十二五”国家科技支撑计划资助项目(2014BAD08B04)和江苏高校优势学科建设工程资助项目(苏政办发\[2014\]37号)
作者单位
付为国 江苏大学 
王凡坤 江苏大学 
李萍萍 南京林业大学 
汤涓涓 江苏大学 
中文摘要:通过不同铅浓度下小白菜根际土壤微生物数量、硝化和反硝化速率及呼吸速率等特征的研究,揭示铅污染对植物根际微生态系统生态安全性的影响。结果表明,随着铅浓度的增加,细菌、真菌和放线菌数量均呈现先升后降的变化趋势,峰值所对应的铅浓度分别为300、300和600mg/kg,3种微生物的耐铅能力为真菌>放线菌>细菌。土壤硝化和反硝化细菌数、硝化和反硝化速率及呼吸速率也随着铅浓度的增加先升后降,各指标峰值所对应的铅浓度均为300mg/kg;硝化速率与硝化细菌数、反硝化速率与反硝化细菌数及土壤呼吸速率与微生物总数决定系数分别为0.6847、0.8511和0.6843,均显著正相关。研究发现,当土壤铅浓度达到或超过1200mg/kg时,小白菜根际微生态系统出现显著的微生物群落结构和功能退化。
Fu Weiguo  Wang Fankun  Li Pingping  Tang Juanjuan
Jiangsu University;Jiangsu University;Nanjing Forestry University;Jiangsu University
Key Words:Brassica chinensis  Microbe  Lead stress  Nitrification  Denitrification  Respiration
Abstract:By studying the characteristics of microbial number, nitrification and denitrification rates and respiration rate of rhizosphere soil of Brassica chinensis L. grown under the different concentration of lead (Pb) environments, the effect of lead on the ecological safety of plant rhizosphere micro-ecosystem was revealed. The results showed that the all numbers of bacteria, fungi and actinomycetes first increased and then decreased with increase of Pb concentration, and the peak value occurred in the environment with soil Pb concentration of 300mg/kg, 300mg/kg and 600mg/kg, respectively. The Pb tolerance of microbe was fungi > actinomycetes > bacteria under the Pb stress environments. The all numbers of nitrifying and denitrifying bacteria in the rhizosphere, soil nitrification and denitrification rate, and soil respiration rate also first increased and then decreased with increase of Pb concentration, and peak values of all above parameters occurred in the environment with soil Pb concentration of 300mg/kg. Nitrification rate and number of nitrifying (R2=0.6847), denitrification rate and number of denitrifying bacteria (R2=0.8511), and respiration rate and the total number of microbe (R2=06843) were all correlated significantly and positively. In conclusion, only when the soil Pb concentrations reaches or exceeds 1200mg/kg, significant microbial community structural and functional degradation of B. Chinensis rhizosphere micro ecosystem will occur.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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