石吉勇,邹小波,张德涛,陈正伟,赵杰文.不同颜色银杏叶总黄酮含量分布高光谱图像检测[J].农业机械学报,2014,45(11):242-245.
Shi Jiyong,Zou Xiaobo,Zhang Detao,Chen Zhengwei,Zhao Jiewen.Determination of Total Flavonoid Content Distribution on Different Color Ginkgo Biloba Leaves[J].Transactions of the Chinese Society for Agricultural Machinery,2014,45(11):242-245.
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不同颜色银杏叶总黄酮含量分布高光谱图像检测   [下载全文]
Determination of Total Flavonoid Content Distribution on Different Color Ginkgo Biloba Leaves   [Download Pdf][in English]
投稿时间:2014-07-02  
DOI:10.6041/j.issn.1000-1298.2014.11.037
中文关键词:  银杏叶 总黄酮 含量检测 高光谱图像
基金项目:国家自然科学基金资助项目(61301239)、江苏省杰出青年基金资助项目(BK20130010)、江苏省自然科学基金资助项目(BK20130505)、中国博士后科学基金资助项目(2013M540422)、中国博士后科学基金资助项目(2014T70483)、江苏省博士后科研计划资助项目(1301051C)和江苏大学高级专业人才科研启动基金资助项目(13JDG039)
作者单位
石吉勇 江苏大学 
邹小波 江苏大学 
张德涛 江苏大学 
陈正伟 江苏大学 
赵杰文 江苏大学 
中文摘要:利用近红外高光谱图像技术研究了总黄酮含量在不同颜色(绿色、黄绿色、黄色)银杏叶片上的二维分布规律。采集120片银杏叶在近红外波段(900~1700nm)下的高光谱图像信息,并利用分光光度计法测定银杏叶片的总黄酮含量;计算高光谱图像中不同波段下的平均灰度作为银杏叶对应的光谱信息,利用逐步线性回归方法建立黄酮含量校正模型(R=0.9307);逐一提取待测银杏叶高光谱图像中每个像素点在不同波段的光谱信息,并将其代入黄酮含量校正模型以计算出各个像素点处对应的黄酮含量,从而绘制总黄酮含量在整个银杏叶片上的二维分布图。研究结果表明,银杏叶总黄酮含量随着绿色、黄绿色、黄色而呈现出递增趋势,且总黄酮含量高的区域主要位于叶片的边缘,总黄酮含量低的区域主要位于叶柄附近。研究为揭示有机组分在农产品、食品中的分布规律提供了技术手段。
Shi Jiyong  Zou Xiaobo  Zhang Detao  Chen Zhengwei  Zhao Jiewen
Jiangsu University;Jiangsu University;Jiangsu University;Jiangsu University;Jiangsu University
Key Words:Ginkgo biloba leaf Total flavonoid content Quantitative analysis Hyperspectral imaging technology
Abstract:Hyperspectral imaging technology was used to determine total flavonoid content distribution map on different color ginkgo biloba leaves (green, green yellowish, yellow). Firstly, 120 Ginkgo biloba leaves were used to collect hyperspectral image data cube and determine total flavonoids concentrations. Secondly, the average gray value of each band in hyperspectral image data cube was calculated as spectal data of Ginkgo biloba leaf. The spectal data and total flavonoids concentrations were used to bulid calibration model (R=0.9307) by using multiple stepwise regression method. Finaly, the calibration model was used to predict the total flavonoid content of each pixel in the hyperspectral image. This enables construction of a distribution map of total flavonoid content on the ginkgo leave. Distribution maps of total flavonoid content in three color groups (green, green yellowish, yellow) were calculated. Results indicate that the level of total flavonoid for green, green yellowish, and yellow were in an increasing order. Higher level of total flavonoids could be noticed at the margin area of the leaves, while lower levels occured at the area near the leafstalk of the ginkgo leaves. This research provided a method to determine major constituent of food and agricultural products.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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