李青林,毛罕平,左志宇,张晓东,孙俊.基于叶片微观结构变化的番茄磷水平检测方法[J].农业机械学报,2014,45(10):282-287.
Li Qinglin,Mao Hanping,Zuo Zhiyu,Zhang Xiaodong,Sun Jun.A Detection Method of Tomato Phosphorus Level Based on Micro-structure of Leaf[J].Transactions of the Chinese Society for Agricultural Machinery,2014,45(10):282-287.
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基于叶片微观结构变化的番茄磷水平检测方法   [下载全文]
A Detection Method of Tomato Phosphorus Level Based on Micro-structure of Leaf   [Download Pdf][in English]
投稿时间:2014-04-07  
DOI:10.6041/j.issn.1000-1298.2014.10.044
中文关键词:  番茄  微结构  磷水平  快速检测
基金项目:国家自然科学基金资助项目(61233006、31201659、31101082)、江苏高校自然科学基金资助项目(11KJB210001)、江苏高校优势学科建设工程资助项目(苏政办发[2014]37号)和江苏大学现代农业装备与技术重点实验室开放基金资助项目(NZ201306)
作者单位
李青林 江苏大学现代农业装备与技术教育部重点实验室 
毛罕平 江苏大学现代农业装备与技术教育部重点实验室 
左志宇 江苏大学现代农业装备与技术教育部重点实验室 
张晓东 江苏大学现代农业装备与技术教育部重点实验室 
孙俊 江苏大学 
中文摘要:以温室番茄为研究对象,借助扫描电镜技术(SEM)和透射电镜技术(TEM)研究了不同磷养分水平下的叶片表面和断面显微结构以及叶肉细胞超微结构变化。结果表明:正常磷水平下的番茄叶片厚度为(124±2.14) μm,表面气孔和绒毛密度分别为(233.0±5.5)个/mm2和(34.2±1.33)根/mm2,绒毛高度为(97.0±2.83) μm,气孔大小为(13.91±0.85) μm。缺磷会造成叶片厚度明显变薄,引起叶片表面气孔密度、绒毛密度和绒毛高度明显降低,并且会破环叶绿体结构的完整性,但对气孔大小的影响不明显;磷过量,会使气孔大小、绒毛密度和维管束数量显著增加,并引起细胞壁厚度增加,但对气孔密度、绒毛高度和叶绿体结构影响不明显。
Li Qinglin  Mao Hanping  Zuo Zhiyu  Zhang Xiaodong  Sun Jun
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, Jiangsu University;Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, Jiangsu University;Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, Jiangsu University;Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, Jiangsu University;Jiangsu University
Key Words:Tomato  Micro-structure  Phosphorus levels  Rapid detection
Abstract:The object of this study was to seek a new way to detect the phosphorus levels of tomato rapidly. Taking the tomato grown under different phosphorus levels as the object of the research, SEM (Scanning Electron Microscope) and TEM(Transmission Electron Microscope) were adopted to evaluate the effects of phosphorus levels on micro-structure and ultra-structure of tomato leaf. The results showed that for the control treatment plant ,the thickness of leaf was (124±2.14) μm, the stomatal and trichome density was (233.0±5.5) mm-2 and (34.2±1.33) mm-2 respectively, the height of trichomes was (97.0±2.83) μm, and the size of the stoma was 13.91±0.85 μm. In comparison with the control treatment, the thickness of leave, the density of stomata, the density and the height of trichomes decreased significantly in response to low phosphorus levels, while the changes in stomata size were not significant. The size of stomata, the density of trichomes, the number of vascular bundle were increased in high phosphorus level plants, while the density of stomata, the length of trichomes did not change markedly. At the ultra-structural level, low phosphorus supplied cell contained chloroplast so that it had begun to lose their structural integrity, and high phosphorus supplied cell had thicker cell wall, but the structure did not change observably. Based on the results, a new method to detect phosphorus levels rapidly was proposed.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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