In order to analyze the flow resistance property of the hemisphere-segment arranged in transverse for the valve less piezoelectric pump with bluff-body and the influence of the hemisphere-segment on the output performance of the pump, the flow resistance action law of multiple hemisphere-segments arranged in transverse in pump chamber was studied. Based on the research of single hemisphere-segment flow resistance and the analysis of influence factors for hemisphere-segments arranged in transverse, the action laws of flow resistance interference coefficient and flow resistance coefficient for two hemisphere-segments arranged in transverse were obtained by experiments. Then the theoretical calculation of transverse flow resistance interference coefficient and flow resistance coefficient for multiple hemisphere-segments was derived. And the experiments on flow resistance and flow rate were carried out by using four hemisphere-segments arranged in transverse. The theoretical derivation was proved to be feasible for analyzing and predicting the flow resistance property and the flow rate of such valve-less piezoelectric pump with bluff body. When the driving voltage was set to 120V and driving frequency was set to 6Hz, the flow rate of the pump reached to 44.78mL/min. And the average deviation between theoretical and experimental was 39.34%.