武丽,李章海,叶文玲,黄义德.钼胁迫对烟草光合荧光参数和叶绿体超微结构的影响[J].农业机械学报,2014,45(8):262-268.
Wu Li,Li Zhanghai,Ye Wenling,Huang Yide.Effects of Photosynthetic Fluorescence Parameters and Chloroplast Ultrastructure of Tobacco under Molybdenum Stress[J].Transactions of the Chinese Society for Agricultural Machinery,2014,45(8):262-268.
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钼胁迫对烟草光合荧光参数和叶绿体超微结构的影响   [下载全文]
Effects of Photosynthetic Fluorescence Parameters and Chloroplast Ultrastructure of Tobacco under Molybdenum Stress   [Download Pdf][in English]
投稿时间:2014-03-24  
DOI:10.6041/j.issn.1000-1298.2014.08.042
中文关键词:  烟草  钼胁迫  光合荧光参数  叶绿体超微结构
基金项目:国家自然科学基金资助项目(41301539)、安徽省教育厅高校重点基金资助项目(KJ2013Z085)和贵州省烟草公司重点科技项目(201105)
作者单位
武丽 安徽农业大学 
李章海 中国科学技术大学烟草与健康研究中心 
叶文玲 安徽农业大学 
黄义德 安徽农业大学 
中文摘要:为探明烟草响应钼胁迫光合生理机制,研究了缺钼胁迫对烟草光合气体交换、叶绿素荧光参数和叶绿体超微结构的影响。结果表明:缺钼培养10d后,烟苗光合色素含量和净光合速率较对照有所降低,胞间二氧化碳浓度、气孔导度和蒸腾速率提高,光系统初始荧光和非光化学猝灭系数提高,光合电子传递速率和最大光化学效率降低,但差异均不显著;烟苗的叶绿体基类片层和基质片层排列松散,淀粉粒数量少。培养20d时,缺钼和对照烟苗的多数指标差异显著。培养30d时,除蒸腾速率外,缺钼和对照烟苗的其它指标差异均显著,叶绿体片层结构受损严重,淀粉粒发育差。缺钼胁迫损伤了叶绿体超微结构,抑制了原初光能转化效率,降低了光合能力。且随胁迫时间延长,缺素表现明显。
Wu Li  Li Zhanghai  Ye Wenling  Huang Yide
Anhui Agricultural University;Research Center of Tobacco and Health, University of Science and Technology of China;Anhui Agricultural University;Anhui Agricultural University
Key Words:Tobacco  Molybdenum stress  Photosynthetic fluorescence parameters  Chloroplast ultrastructure
Abstract:With the aim to investigate the physiological mechanism of tobacco response to molybdenum stress, the effect of photosynthetic pigment, gas exchange parameters,chlorophyll fluorescence parameters and chloroplast ultrastructure of tobacco were studied under molybdenum deficiency stress by the hydroponic experiment. The results showed that, compared with the control group, the photosynthetic pigment contents and net photosynthetic rate of tobacco leaves cultivated about 10d decreased, while intercellular CO2 concentration, stomatal conductance and transpiration rate increased. And the initial fluorescence and non-photochemical quenching coefficient (NPQ) of PS II increased, while photosynthetic electron transport rate (ETR) and the maximal photochemical efficiency decreased,but the differences between two treatments were not significant. The grana layer of the chloroplast ultrastructure under molybdenum deficiency stress expanded to deformation and loose,the number of amyloid was less and it grew worse. The most indicators between molybdenum deficiency and control tobacco cultivated about 20d were significant. In addition to Tr, other indexes between molybdenum deficiency and control tobacco cultivated about 30d were significant, and the chloroplast under the molybdenum deficiency stress was damaged seriously. Therefore,under the molybdenum deficiency stress,the chloroplast ultrastructure grows worse, the activity of PS II is inhibited, and the Pn decreases. With the extension of molybdenum stress time, the deficiency reaction is more obvious.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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