文鹏程,王军,任发政,韩玲,王琳琳,纪银莉.牧区奶干渣组合式发酵剂响应面法优化[J].农业机械学报,2014,45(8):241-247.
Wen Pengcheng,Wang Jun,Ren Fazheng,Han Ling,Wang Linlin,Ji Yinli.Optimization of Combined Fermentation Starter of Milk Dried Residue in Pastoral Areas by Response Surface Methodology[J].Transactions of the Chinese Society for Agricultural Machinery,2014,45(8):241-247.
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牧区奶干渣组合式发酵剂响应面法优化   [下载全文]
Optimization of Combined Fermentation Starter of Milk Dried Residue in Pastoral Areas by Response Surface Methodology   [Download Pdf][in English]
投稿时间:2013-12-03  
DOI:10.6041/j.issn.1000-1298.2014.08.039
中文关键词:  奶干渣  乳酸菌  响应面  滴定酸度
基金项目:公益性行业(农业)科研专项经费资助项目(201303085)和国家国际科技合作专项资助项目(2011DFA32550)
作者单位
文鹏程 甘肃农业大学 
王军 甘肃农业大学 
任发政 中国农业大学 
韩玲 甘肃农业大学 
王琳琳 甘肃农业大学 
纪银莉 甘肃华羚生物技术研究中心 
中文摘要:以脱脂牦牛乳为试验材料,发酵3.5h滴定酸度为试验指标,在不同菌种最佳组合方式筛选的研究基础上,利用响应面Box-Behnken中心组合试验研究了4株筛自牧区、发酵性能优良的菌种:保加利亚乳杆菌(MGD1-3)、嗜热链球菌(MGB39-5)、嗜热链球菌(G81-1)、植物乳杆菌(BM5152)不同接种比例对牧区奶干渣发酵效果的影响。结果表明,优化得到最优组合式发酵剂为:MGD1-3体积分数3.06%、MGB39-5体积分数0.64%、G81-1体积分数0.55%、BM5152体积分数2.04%,滴定酸度为75.87°T。为了便于实际操作,确定最终体积接种比为:MGD1-3∶MGB39-5∶G81-1∶BM5152=30∶6∶5∶20,接种量体积分数为6.0%,该条件下进行3次平行试验,得到滴定酸度平均值为74.21°T;将接种优化后组合式发酵剂制成的奶干渣(试验组)与牧区采集奶干渣(对照组)进行感官与理化指标比较,结果表明该组合式发酵剂发酵性能优良。
Wen Pengcheng  Wang Jun  Ren Fazheng  Han Ling  Wang Linlin  Ji Yinli
Gansu Agricultural University;Gansu Agricultural University;China Agricultural University;Gansu Agricultural University;Gansu Agricultural University;Gansu Hualing Bio-technical Research Center
Key Words:Milk dried residue  Lactic acid bacteria  Response surface design  Titratable acidity
Abstract:Skimmed yak milk was selected for this research and the titratable acidity at 3.5h as the test indicator. Box-Behnken central composite experiment was designed based on optimal combination of fermentation starters to research the effect of Lactobacillus delbrueckii subsp bulgaricus(MGD1-3), Streptococcus thermophilus(MGB39-5), Streptococcus thermophilus(G81-1), and Lactobacillus plantarum(BM5152), which were screened from pastoral areas to the titratable acidity. The result showed that the optimal combination of fermentation starters was MGD1-3 3.06%, MGB39-5 0.64%, G81-1 0.55%, and BM5152 2.04% and the titratable acidity was 75.87°T. In order to facilitate the practical operation, the proportion of MGD1-3∶MGB39-5∶G81-1∶BM5152=30∶6∶5∶20 and 6.0% of inoculum size based on volume fraction were selected. Under these conditions, the average titratable acidity was 74.21°T of three parallel trials. Compared with the sensory and physical and chemical indicators of milk dried residue came from optimized combination of fermentation starters and pastoral areas, the result showed that the optimized combination of fermentation starters was reliable.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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