Effect of independently developed microbial inoculum on cattle manure enhanced composting was studied, which was named CC-1 with high lignocellulose degradation ability. CC-1 was a mixture with Geotrichum candidum-BD1, Streptomyces thermocarboxydus-SM, Bacillus subtilis-KC and Coriolus versicolor, in which the first three kinds of microbial were selected from cattle manure in Beijing areas. The orthogonal composting test was conducted in simulated fermentation tanks to study the effects of different parameters such as C/N ratio(26, 28, 32), initial water content(55%, 60%, 65%), aeration rate (10min, 20 min, 30min ventilation every day, with 1.63m3/min ventilation pump power), and inoculation amount of microbial agents on cattle manure composting. And composting effect was evaluated from temperature changes, C/N, sensory effect (including material particle size and stench) and total nutrients contents. The results showed that the optimum control parameters were as follows: C/N was 28, water content of compost materials was 55%, microbial inoculation amount was 0.8%(volume ratio) and 20 minutes ventilation every day (ventilation volume was 5.09m3/(min·m3)). Then, with the optimum conditions, another composting was carried out to prove the effect of CC-1 on cattle manure composting, and the composting inoculated by EM and composting with non inoculum were taking as the controls. In this test, temperature changes and lignocellulose degradation during composting were measured. Besides, pH value, GI (germination index), TC, TN, TP, TK, and humic acid were determined when composting finished. The results showed that the composting period was shortened. The period of temperature rising to 50℃was 2 or 3 days earlier and the period of temperature decreasing to 40℃ below was 8 days earlier, compared to that inoculated by EM and non inoculated. When composting finished, pH value and GI of compost inoculated by CC-1 were 7.9 and 106.5%. Compared to compost that inoculated by EM and non-inoculated, the TN was 19.33% and 27.35% higher, total nutrient was 7.91% and 23.95% higher, and the humic acid was 16.38% and 47.53% higher in compost that inoculated by CC-1. Degradation ratios of hemi-cellulose and cellulose in compost inoculated by CC-1 was 1.2 and 1.8 times, and 1.8 and 2.1 times that in compost inoculated by EM and non-inoculated, respectively.