季方,郝睿,刘颖,李道义,周宇光,董仁杰.一株高生物量链带藻分离筛选与培养条件优化[J].农业机械学报,2013,44(Supp2):149-154.
Ji Fang,Hao Rui,Liu Ying,Li Daoyi,Zhou Yuguang,Dong Renjie.Optimization of Environmental Factors for Biomass Production of Isolated Strain of Desmodesmus sp. EJ12-3[J].Transactions of the Chinese Society for Agricultural Machinery,2013,44(Supp2):149-154.
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一株高生物量链带藻分离筛选与培养条件优化   [下载全文]
Optimization of Environmental Factors for Biomass Production of Isolated Strain of Desmodesmus sp. EJ12-3   [Download Pdf][in English]
  
DOI:10.6041/j.issn.1000-1298.2013.S2.028
中文关键词:  链带藻 分离筛选 生物量累积 培养条件 响应面分析
基金项目:“十二五”国家科技支撑计划资助项目(2012BAD47B03)、国家高技术研究发展计划(863计划)资助项目(2013AA065802)、中国博士后科学基金面上资助项目(2011M500451)、中央高校基本科研业务费专项资金资助项目(2013YJ007)和生物质工程交叉学科北京市重点学科建设项目
作者单位
季方 中国农业大学工学院 
郝睿 农业部可再生能源清洁化利用技术重点实验室 
刘颖 中国农业大学工学院 
李道义 中国农业机械化科学研究院 
周宇光 中国农业大学工学院 
董仁杰 中国农业大学工学院 
中文摘要:为了得到具有高生物量累积特性的微藻及其最优培养条件,从自然水样中分离筛选出一株生物量较高的微藻属,经18s rRNA及ITS1鉴定为链带藻属(Desmodesmus sp.),命名为EJ12-3。通过单因素试验及响应面分析对其培养条件进行优化,得到最大生物量累积的条件为:温度28℃,光照强度131 μmol/(m2·s),光周期15∶9 (光暗时间比),pH值为6,在此条件下,其培养14 d后的生物量累积可达(0.648±0.015) g/L。并得到这些环境因素对链带藻EJ12-3生长的交互影响,得到二次方程模型,该模型的拟合较好(R2为0.950),有一定的应用价值。
Ji Fang  Hao Rui  Liu Ying  Li Daoyi  Zhou Yuguang  Dong Renjie
College of Engineering, China Agricultural University;Key Laboratory of Clean Production and Utilization of Renewable Energy, Ministry of Agriculture;College of Engineering, China Agricultural University;Chinese Academy of Agricultural Mechanization Sciences;College of Engineering, China Agricultural University;College of Engineering, China Agricultural University
Key Words:Desmodesmus sp. Isolation Biomass accumulation Culture conditions Response surface methodology
Abstract:With the aim of isolating a high biomass accumulation strain and optimize environmental factors to enhance its biomass production, an unicellular green algae was isolated from fresh water samples, the morphological and genomic characterization identification of this strain was carried out by using 18s rRNA and ITS1 analysis. This newly isolated strain named EJ12-3 was identified as Desmodesmus sp. The environmental factors for biomass production of Desmodesmus sp. EJ12-3 was optimized by using response surface methodology (RSM). The experimental and the predicted values were very close which reflected the accuracy and the applicability of RSM (R2=0.950). According to the results, the optimal condition for biomass production within the experimental range of the variables studied was: temperature of 28℃, light intensity of 131 μmol/(m2·s), light/dark cycle of 15∶9 and pH value of 6.0. Under this condition, the predicted biomass production value was up to (0.648±0.015) g/L.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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