王向阳,王红艳.不同离心强度损伤对绿豆芽抗病生理的影响[J].农业机械学报,2013,44(12):198-203,214.
Wang Xiangyang,Wang Hongyan.Effects of Different Centrifugal Intensity on Disease Resistance of Mung Bean Germinations[J].Transactions of the Chinese Society for Agricultural Machinery,2013,44(12):198-203,214.
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不同离心强度损伤对绿豆芽抗病生理的影响   [下载全文]
Effects of Different Centrifugal Intensity on Disease Resistance of Mung Bean Germinations   [Download Pdf][in English]
  
DOI:10.6041/j.issn.1000-1298.2013.12.033
中文关键词:  绿豆芽  机械伤害  抗病性  离心强度
基金项目:浙江省重大科技专项资助项目(2011C12031)和浙江工商大学校级创新基金资助项目(1110XJ511105)
作者单位
王向阳 浙江工商大学 
王红艳 浙江工商大学 
中文摘要:对绿豆芽(Vigna radiata)进行了可逆损伤(94g)、轻微损伤(376g)、中度损伤(845g)、严重损伤(1503g)和破损损伤(3381g)的不同离心强度(加速度)的损伤,g为重力加速度,取9.8m/s2,测定过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性以及超氧阴离子自由基(O-2)的产生速率、过氧化氢(H2O2)质量摩尔浓度、水杨酸(SA)质量浓度和木质素含量变化。包括可逆损伤在内的所有损伤,在损伤初期都会诱导抗病生理。可逆损伤的游离态SA质量浓度、H2O2质量摩尔浓度和POD活性显著上升,O-2的产生速率、总酚质量浓度、木质素含量、SOD活性、CAT活性、PAL活性和PPO活性变化不显著;轻微损伤、中等损伤、严重损伤引起更多抗病生理指标显著变化,特别是轻微和中等损伤的POD活性上升最为显著;破损损伤的游离态SA质量浓度、H2O2、总酚、木质素含量上升最大,但是O-2产生速率、CAT、PAL、PPO活性下降也最大。SOD活性对损伤敏感性较低。
Wang Xiangyang  Wang Hongyan
Zhejiang Gongshang University;Zhejiang Gongshang University
Key Words:Mung bean germination  Mechanical damage  Disease resistance  Centrifugal intensity
Abstract:The Catalase(CAT), Peroxidase(POD), Superoxide dismutase(SOD), Polyphenol oxidase(PPO)and Phenylalnine ammonialyase(PAL)activity, the Superoxide anion free radical(O-2)generation rate, the Hydrogen peroxide(H2O2), Salicylic acid and Lignin content of mung bean germinations were detected, which were subjected to different centrifugal intensity (acceleration), such as reversible damage(94g), slight damage(376g), moderate damage(845g), serious damage(1503g), and breakage damage(3381g). One gravitational acceleration (g)=9.8m/s2. The results showed that the disease resistance would be induced by all the centrifugal damages including reversible damage in the early stage. The content of free Salicylic acid(SA) and H2O2, POD activity were significantly increased by reversible damage while the change of the O-2 generation rate, total hydroxybenzene, lignin content, the SOD, CAT, PAL and PPO activity were indistinctive. The significant changes of more disease resistance indicators were caused by slight damage, moderate damage and serious damage. Especially the POD activity was increased remarkably under slight damage and moderate damage. The content of free SA, H2O2, total hydroxybenzene and lignin rose in the maximum while the O-2 generation rate, the CAT, PAL and PPO activity declined in the maximum when it was treated with breakage damage. Besides, the SOD activity was not sensitive to all the damages. 

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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