A crop canopy 3-D reconstruction and volume measurement method that based on the ultrasonic sensing technology was proposed. The functions of interp1(), quad and surf in Matlab were introduced for crop canopy description and the volume calculation. Indoor measurements were conducted on six ridges of greenhouse strawberry by 3 times repeats and the results were compared with manual measurement. The result showed the experiments presented good repeatability（R2 was 0.9275, RMSE was 0.1358m3), which showed consistent with the manual measurement (R2 is 0.9411, RMSE was 0.1345m3). Therefore, the proposed measuring system was stabilized and feasible.