Accurate detection of agro-product quality can provide reliable basis for postharvest preservation, determining when to sell, predicting shelf-life and sorting. Laser Doppler vibrometer (LDV) technology is based on the Doppler-effect sensing the frequency shift of back scattered light from a moving surface, and has the advantages of high sensitivity, non-contact measurement, avoiding mass loading implication and so on. This technology can be used for nondestructive evaluation of agro-product quality by inspecting its vibrational properties. The development and principle of LDV technology, and its applications in nondestructive detection of agro-product quality were introduced.