The tractor navigation system based on difference GPS and PDA was designed, and both integrated wireless bluetooth and TCP/IP were employed for transmitting data between GPS receiver and PDA. Windows mobile operating system was installed in PDA, on which the test program was designed resort to LabVIEW and its mobile module. The test program was constructed and managed by using state machine and queue methods, where the path, velocity, heading angle and positioning accuracy were displayed in real time while testing. In the end of sampling, data playback and packet dropout checking were conducted. A roadway test system was developed. The high bluetooth bus load experiments under the tractor’s electromagnetic circumstance were conducted, which showed that the wireless communication of bluetooth worked properly, and there were no clogging and packet loss during transmitting data while increasing GPS output statement speed to more than 1 Mb/s. Finally, the systematic tractor ground experiments also indicated the data wireless transfer stability and reliability. 