As one of the most important optical nondestructive detection technologies, hyperspectral imaging technology was widely used for assessing for agro-product quality and safety. At first, the advancement on hyperspectral imaging technology of agricultural and livestock products quality and safety was reviewed. The research and application of this technology in fruits and vegetables are wide, including internal quality detection, such as nutritional component analysis, eating quality evaluation and real time online quality classification; external quality detection that consists characteristics identification, surface defect and chilling injury inspection; and detection of pesticides residue. In respect of fresh meet quality and safety detection, the hyperspectral imaging technology was investigated to develop application technologies and inspection equipments, including real-time analysis of nutritional components, online evaluation of meat eating quality parameters such as tenderness, marbling and freshness, and rapid evaluation of meat safety attributes such as microbial contamination and bacteria total viable count. Finally, the status and issues of hyperspectral technology were analyzed. Its development trend prospects was presented in the nondestructive detection of agricultural and livestock products.