Bionic structure of jute fiber was designed. The mechanical properties of jute fiber with bionic structure were tested. First, jute fiber was divided into filaments. The surface treatment was used on jute fiber filaments: three shares of jute fiber were woven into a different helix angles bionic helical structure by using the spiral knitting machine and stereotypes. The size of the helix angle could be controlled by adjusting the speed of the spiral knitting machine. Its mechanical properties were tested. Results showed that, the breaking load of jute fiber which had bionic helical structure first increased and then decreased. Spiral fiber elongation was declined with the decrease of helix angle. Jute fiber with bionic helical structure had optimum comprehensive mechanical properties at the helix angle of 66°. When the helix angle was more than 66°, the stress of the fiber was unbalance, and then the fiber was fractured. When the helix angle is less than 66°, the fiber force increased with the decreasing of helix angle’s.