The beef-marbling detecting system combined with spectral and computer vision technology was created. Working principle, hardware structure, choice of hardware parameters and the software of the detecting system ware introduced. Center wavelength of the filter used in this detecting system was 530nm, and the image of sample under this wavelength was taken by an industrial camera. Image processing software used to detect beef-marbling was developed. The results of experiment show that detect accuracy of the system can match the demand of beef-marbling detect.