Under the aeroponics cultivation experiment of the different levels of Fe, the relative physiological indexes of the potato minituber was tested. The effect of the different concentrations of Fe ion on relative physiological indexes of the potato minituber was analyzed to explore reasonable application of Fe for improving the quality and yield of potato minituber. The results showed that iron content would affect the synthesization of chlorophyll a and b. Protein contents decreased in a certain extent. Photosynthesis and the accumulation of photosynthetic products decreased. The experiment validated that the unsuitable application of Fe reduced the quality and yield of potato minituber. The suitable Fe ion concentration of nutrient solution in cultivation of the potato minituber with aeroponics method in greenhouse was put forward. 