The non-volatiles from clove were fractionated by liquid-liquid partition by using ethyl acetate, n-butanol and water, respectively. The antioxidant activity of three different fractions was evaluated by using different antioxidant tests, including total antioxidant activity, superoxide radical, hydroxyl radicals scavenging activities and inhibition of peroxidation of linoleic acid. In the above four assays, ethyl acetate fraction exhibited more antioxidant potency than other fractions and was effective fraction. Correlation analysis indicated that there was a linear relationship between antioxidant activity and the content of phenolic compounds. Through the use of thin-layer chromatography (TLC), there were eight major constituents in the effective fraction. Furthermore, the effective fraction was found to be more effective by acid hydrolysis in total antioxidant activity, hydroxyl radicals and superoxide radical scavenging activity.