For studying the separation equipment for rind and pith of corn straw, the key mechanism for separation of rind and pith was experimented. In experiments, distance between pith-stripping blades and supporting blade, quantity and installation angle of pith-stripping blades, rotation speed of pith-stripping roll were chosen as experimental factors. Rind-intact percentage and pith-remain percentage were decided as evaluation indexes. The results showed that with distance of 2.0～3.0 mm, quantity of blades of 6 pieces, installation angle of blades of 15°and rotation speed of roll of 1 000 r/min, rind-intact percentage was over 95% and pith-remain percentage was below 2%. 