Acinetobacter lwoffi was studied by impedance and plate count method throughout rotational combination design to define the effects of temperature and pH value on maximal specific growth rate (μm) of A. lwoffi, conductance and admittance. Polynomial models were established (Pr<0.05) and validated. The results showed that the established model with viable count method was better than the other two (R2=0.9455), which fit well when μm was less than 0.06. The correlation equation between μm of conductance/admittance and μm of A. lwoffi were established and the R2 were 0.859 and 0.806, respectively.