The optimum extraction technology and antioxidant activity of glycoprotein from Camellia oleifera seed were performed for future development and utilization of the Camellia oleifera. On the base of singlefactor experiment, the optimum parameters of extraction for glycoprotein from Camellia oleifera seed were determined by response surface methodology. The antioxidant activity of glycoprotein was examined by using various antioxidant assays with vitamin C co-assayed as positive control. As a result, the optimized condition of glycoprotein extraction was as follows: extraction time of 8.81h, salinity of 0.12mol/L, pH value of 8.77, ratio of liquid to solid of 11.62mL/g. At the optimum conditions, the yield of protein reached to 8.76%, and the yield of saccharide reached to 10.14%. The results of above antioxidant tests demonstrated that the glycoprotein from Camellia oleifera seed has certain antioxidant activities.