To further study mechanism on the processing tomato separation, separation device vibrator movement was analyzed. Its theoretical model by using dAlembert principle was established. Virtual prototype movement based on SolidWorks was simulated. Separation roller motion process was filmed by highspeed camera on homemade FS-35 type separation test rig and the test video was analyzed by blasting software Blaster’s Mas. The simulation results and test analysis showed separation unit movement followed the rule of theoretical model, which verified the correctness of the theoretical derivation.