何荣海,马海乐,余筱洁.条斑紫菜ACEI抑制肽的超滤分离与离子交换纯化[J].农业机械学报,2012,43(2):146-150,204.
He Ronghai,Ma Haile,Yu Xiaojie.Ultrafiltration and Ionic Exchange Purification of ACE Inhibitory Peptides from Hydrolysate of Porphyra yezoensis Protein[J].Transactions of the Chinese Society for Agricultural Machinery,2012,43(2):146-150,204.
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条斑紫菜ACEI抑制肽的超滤分离与离子交换纯化   [下载全文]
Ultrafiltration and Ionic Exchange Purification of ACE Inhibitory Peptides from Hydrolysate of Porphyra yezoensis Protein   [Download Pdf][in English]
  
DOI:10.6041/j.issn.1000-1298.2012.02.029
中文关键词:  条斑紫菜  ACEI抑制肽  超滤  离子交换
基金项目:中国博士后科学基金资助项目(20100471386)、江苏省博士后科学基金资助项目(0902029C)、浙江省自然科学基金资助项目(Y3110025)、江苏高校优势学科建设工程资助项目、南通市产学研联合创新科技计划资助项目(B2010008)和江苏大学高级人才科研启动基金资助项目(08JDG032)
作者单位
何荣海 江苏大学 
马海乐 江苏大学 
余筱洁 浙江农林大学 
中文摘要:对条斑紫菜ACEI抑制肽进行了超滤分离和离子交换纯化的试验研究。先后采用截留分子量分别为10 kDa和3 kDa的超滤膜对条斑紫菜ACEI抑制肽超滤,分子量小于3 kDa的组分对ACE活性抑制最高,多肽的IC50值从超滤前的0.844 mg/mL下降到0.523 mg/mL。南开D61阳离子树脂对分子量小于3 kDa的ACEI抑制肽组分吸附率最大,用不同浓度氨水阶梯洗脱,得到第2个洗脱峰的蛋白回收率和活性回收率最高,分别为45.2%和61.5%,其IC50值最低,为0.127 mg/mL,比纯化前下降了75.7%。
He Ronghai  Ma Haile  Yu Xiaojie
Jiangsu University;Jiangsu University;Zhejiang A & F University
Key Words:Porphyra yezoensis, ACE inhibitory peptides, Ultrafiltration, Ionic exchange
Abstract:Angiotensin I-converting enzyme (ACE) inhibitory peptides from digested Porphyra yezoensis protein were purified with ultrafiltration and ionic exchange. The hydrolysate of Porphyra yezoensis protein was ultrafiltered by 10 kDa and 3 kDa membrane in sequence. It was found that the IC50 value of Porphyra yezoensis peptides decreased from original 0.844 mg/mL to 0.523 mg/mL. And Nakai D61 cation resin was selected according to its largest absorption in the ionic exchange static absorption test. The results showed that the second elution peak had the highest protein recovery, ACE inhibitory activity recovery and the lowest IC50 value, which were 45.2%, 61.5% and 0.127 mg/mL, respectively. The sample’s IC50 value decreased by 75.7% after the ionic exchange purification.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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