刘长虹,蔡路昀,韩晓旭,应铁进.UV-C照射后番茄差异表达基因的基因芯片检测[J].农业机械学报,2012,43(1):134-140.
Liu Changhong,Cai Luyun,Han Xiaoxu,Ying Tiejin.Microarray Analysis of Gene Expression Profile Induced by Postharvest UV-C Irradiation in Tomato Fruit[J].Transactions of the Chinese Society for Agricultural Machinery,2012,43(1):134-140.
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UV-C照射后番茄差异表达基因的基因芯片检测   [下载全文]
Microarray Analysis of Gene Expression Profile Induced by Postharvest UV-C Irradiation in Tomato Fruit   [Download Pdf][in English]
  
DOI:10.6041/j.issn.1000-1298.2012.01.024
中文关键词:  番茄  储藏  短波紫外线  基因表达
基金项目:国家自然科学基金资助项目(30972036)
作者单位
刘长虹 浙江大学 
蔡路昀 浙江大学 
韩晓旭 浙江大学 
应铁进 浙江大学 
中文摘要:应用Affymetrix基因芯片研究了4kJ/m2短波紫外线(UV-C)照射后7d番茄总基因表达谱的变化情况。结果表明,UV-C照射后7d,5177个基因在杂交后发生变化,差异表达变化倍数值在大于2和小于-2范围内基因共481个,其中上调基因331个,下调基因150个。上调基因主要包括细胞壁相关基因、信号转导基因、胁迫相关基因和抗性基因等;下调基因主要包括乙烯相关基因和初级代谢基因等。研究结果为探索采后UV-C照射诱导番茄抗性反应、抑制乙烯合成,从而推迟成熟、延长番茄采后寿命的分子机制提供了基础。
Liu Changhong  Cai Luyun  Han Xiaoxu  Ying Tiejin
Zhejiang University;Zhejiang University;Zhejiang University;Zhejiang University
Key Words:Tomato fruit  Storage  UV-C  Gene expression
Abstract:To obtain an overall view on transcript modification during the tomato fruit in response to postharvest UV-C irradiation (4kJ/m2), a microarrary analysis was performed by using Affymetrix tomato genechip arrays. The results showed that 331 and 150 genes were up- or down-regulated, respectively, more than two folds in postharvest UV-C irradiated tomato fruit as compared with that in control fruit. The up-regulated genes mainly involved cell wall related, signal transduction, stress related and defense response. Conversely, genes related to ethylene and primary metabolism were generally down-regulated. These results opened ways to probe into the molecular mechanisms of the effects of postharvest UV-C irradiation on increasing disease resistance, delaying ripening and prolonging postharvest life in tomato fruit.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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