Focusing on the problems existing in the biochip microarray manufacturing methods such as low maintenance in bioactivity, microjet head blockage and high consumption in biological reagent and so on, the ultrasonic transducer and spherical acoustic lens based on ultrasonic focusing microjet head as well as the downward isolated jetting mechanism was designed. Based on this, no nozzle, non blockage, non contact, low consumption and high accuracy was gained in the microarray manufacture. To verify the biocompatibility of the microjet system, the F0F1—ATP molecular-motor assembled liposome microarrays with regular shape and accurate location was prepared. Furthermore, the ATP hydrolysis activity and the ATP synthesis activity of the liposome microarrays were tested respectively. Experimental results indicated that the liposome microarrays fabricated this way showed favorable ATP hydrolysis activity and synthesis activity, which confirmed that the ultrasonic focusing microjet method has favorable biocompatibility in manufacturing biological microarrays. The investigation elaborated here explored a new manufacturing approach for biological microarrays.