Extraction method of Changshanhuyou peel total flavonoids by supercritical carbon dioxide fluid was studied. On the basis of entrainer test and extraction pressure, extraction temperature, dynamic extraction time, CO2 flow rate, orthogonal experiment was carried out. The results showed that entrainer was unnecessary and the optimum extraction pressure was 35MPa, extraction temperature was 45℃, extraction time for dynamic extraction was 60min after static extraction for 40min, and the flow rate of CO2 was 3L/min. Under above process conditions, the extraction yield of Changshanhuyou peel total flavonoids reached to the maximum of 1.01mg/g.