In view of the special structure of bamboo, bamboo OSB flaker was developed. Development concept, mechanical and hydraulic design were presented. Then bamboo OSB flaker was tested run. Bamboo flake processed by bamboo OSB flaker was pressed into bamboo OSB, and the main physical and mechanical properties were tested and researched. The results indicated that bamboo flake with high quality shape could be sliced, which made the even distribution of outer of bamboo in the bamboo flake. Moreover, the bamboo OSB flaker has the characteristics of high efficiency, minor impact vibration and etc. The tests show that the key performance indicators of bamboo OSB are higher than the standards of LY / T 1580-2000 (OSB/4). Therefore, the research and design of bamboo OSB flaker is reasonable and effective. 