王丽波,赵玉,徐雅琴.南瓜多糖大孔吸附树脂纯化工艺[J].农业机械学报,2010,41(9):138-142.
.Purification Technology of Pumpkin Polysaccharides with Macroporous Adsorption Resin[J].Transactions of the Chinese Society for Agricultural Machinery,2010,41(9):138-142.
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南瓜多糖大孔吸附树脂纯化工艺   [下载全文]
Purification Technology of Pumpkin Polysaccharides with Macroporous Adsorption Resin   [Download Pdf][in English]
  
DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]
中文关键词:  南瓜多糖  大孔吸附树脂  分离纯化
基金项目:
王丽波  赵玉  徐雅琴
东北农业大学
中文摘要:为提高南瓜多糖的纯度,筛选适宜南瓜多糖纯化的大孔树脂,确定纯化工艺参数,选用9种大孔树脂材料,采用静态与动态吸附-解吸方法对南瓜多糖进行纯化,以吸附量与解吸率为考察指标对树脂进行选择与工艺研究。结果表明:D101-Ⅰ型大孔树脂更适宜作为南瓜多糖纯化的树脂;其纯化最佳工艺条件为:上柱液质量浓度4.79mg/mL,上柱液体积3.2BV,上柱流速3BV/h,洗脱液乙醇体积分数25%,洗脱速度4BV/h,洗脱液体积3.6BV;上述纯化工艺使南瓜多糖纯度从
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Abstract:25.68%提高到61.39%。In order to select suitable macroporous resin, to improve the purity of pumpkin polysaccharide and to determine purification process parameters, 9 kinds of macroporous resin were chosen to carry out static and dynamic adsorption-desorption experiments. With the indexes of adsorption capacity and elution rate, the suitable macroporous resin was selected and process parameters were confirmed. The results show that D101-I resin has the best performance of adsorption and desorption effects for purification of pumpkin polysaccharide, the optimum technology parameters are sample concentration of 4.79mg/mL, sample volume of 3.2BV, flow rate of 3BV/h, 25% ethanol for elution, elution velocity of 4BV/h, elution volume of 3.6BV. The purity of pumpkin polysaccharide increases from 25.68% to 61.39% by using the above technology. 

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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