马海乐,耿静静,骆琳,杨巧绒,朱文学.超声波预处理对燕麦蛋白制备ACE抑制肽的影响[J].农业机械学报,2010,41(9):133-137.
.Oat-seed Protein Pretreatment by Ultrasonic on Preparation of ACE Inhibitory Peptides[J].Transactions of the Chinese Society for Agricultural Machinery,2010,41(9):133-137.
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超声波预处理对燕麦蛋白制备ACE抑制肽的影响   [下载全文]
Oat-seed Protein Pretreatment by Ultrasonic on Preparation of ACE Inhibitory Peptides   [Download Pdf][in English]
  
DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]
中文关键词:  燕麦蛋白  ACE抑制肽  超声波  预处理  酶解
基金项目:
马海乐  耿静静  骆琳  杨巧绒  朱文学
江苏大学
中文摘要:利用燕麦蛋白制备ACE抑制肽时先利用超声波对燕麦蛋白原料进行预处理研究。以ACE抑制活性和水解度为指标,考察了超声波功率、处理时间、超声波工作/间歇时间对燕麦蛋白预处理效果的影响。结果表明,超声波预处理的最佳工艺参数为:超声波功率500W,处理时间20min,超声波工作时间2 s、间歇时间2 s,该条件下酶解产物对ACE的半抑制浓度IC50值从0.533mg/mL降到0.299mg/mL,酶解时间由常规酶解的90 min缩短到60 min;超声波预处理参数变化虽然对水解度影响不显著,但对ACE抑制活性的影响显著;验证了超声波引起的蛋白疏水性增加是导致酶解产物ACE抑制活性显著改善的重要原因之一。
Key Words:
Abstract:In order to improve enzymolysis technology for preparation of angiotensin-converting enzyme(ACE) inhibiting peptides from oat-seed protein by alcalase protease, ultrasonic pretreatment of oat-seed protein solution was investigated. Based on the inhibitory activity of ACE and degree of hydrolysis (DH), the effects of ultrasonic power, pretreatment time and the on-off time ratio on ultrasonic pretreatment efficacy were studied. The results show that the optimum conditions of ultrasonic pretreatment are 500W of ultrasonic power, 20min of pretreatment time and 2s/2s of ultrasonic on-off time ratio. Under the optimum conditions, half inhibiting concentration (IC50) value of ACE decreases from 0.533mg/mL to 0.299mg/mL, and the time of enzymolysis reduces from 90min to 60min. Experiments also show that ultrasonic pretreatment parameters have made a significant impact on ACE inhibitory activity while no significant effect on the DH. It is verified that the increase of protein hydrophobicity caused by ultrasound is one of main reasons for significant improvement of hydrolysates ACE inhibitory activity. 

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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