De-coloration rate, polysaccharides holding rate and protein removal rate of eight resins on longan polysaccharides were studied. The results showed that anion exchange resin D301-F presented good de-colorizing effects. De-colorizing experiments were carried out by single experiment and orthogonal experiment. The data indicated that the colorants were most possibly small-molecular-weight substances with negative charge. The optimum technological parameters were as follows: the ratio for resin and crude polysaccharides solution (the mass ratio was 4%) was 0.16g/mL under the condition of 50℃ and pH value 5. The de-coloration rate was 90.21%, the polysaccharides holding rate was 85.75%, the protein removal rate was 73.12%. 