Based on the flow characteristics of diffuser/nozzle used in valve-less piezoelectric pump, a new type of “V”-shape tube was presented for micro total analysis systems. The structure and flow property of the valveless piezoelectric pump with “V”-shape tube was analyzed. Finite element method was applied to simulate the flow in the “V”-shape tube for the flow characteristics. And the ANSYS was employed to analyze numerically the effects of the “V”-shape tube structure on the flow resistance property . The results show that, compared with the splitter angle, diffuser angle and the inlet width of “V”-shape tube, the effect of the length of the “V”-shape tube on the flow resistance property is smaller. And a large depth benefits to improve the pump efficiency. 