隋欣,任发政,鲁言文,陈历俊.热激法修饰瑞士乳杆菌的参数优化与特性研[J].农业机械学报,2009,40(8):111-114.
.Optimization and Properties for Lactobacillus helveticus by Heat-shock Treatment[J].Transactions of the Chinese Society for Agricultural Machinery,2009,40(8):111-114.
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热激法修饰瑞士乳杆菌的参数优化与特性研   [下载全文]
Optimization and Properties for Lactobacillus helveticus by Heat-shock Treatment   [Download Pdf][in English]
  
DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]
中文关键词:  瑞士乳杆菌  热激处理  特性  微观结构  参数优化
基金项目:
隋欣  任发政  鲁言文  陈历俊
中国农业大学
中文摘要:采用不同热处理温度(67、70℃)与时间(10、15、20s)对瑞士乳杆菌(Lactobacillus helveticus)进行热激修饰,研究处理前后与干酪加工相关的菌体特性,同时对热激修饰条件参数进行优化。研究结果表明,菌体经70℃、10?s热激处理后,菌体存活率、动态产酸能力、蛋白水解能力等指标符合热激要求,经修饰后的菌株适宜作为干酪促熟酶源。采用扫描电镜对细菌表面微观结构的观察结果表明,热激处理使菌体形态、膜表面发生明显改变,菌体表面发生萎缩、褶皱与底端溶解等变化。
Key Words:
Abstract:The effects of different heat-shock treatments on biochemical properties and microstructure of Lactobacillus helveticus were determined. The strain was subjected to heat-shock treatment at 67℃ and 70℃, holding time was 10s, 15s and 20s, respectively. The biochemical properties related to cheese ripening such as cell residual viability, lactic acid productivity, and proteolysis activity were monitored. The results suggested that the modified cells using heat-shock parameter of 10s at 70℃, gave the best results upon inhibiting lactic acid productivity and the remaining proteolysis activity. Moreover, SEM photographs showed the changes of surface structure on cell membranes, and elucidated the relationship with heat-shock treatment.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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