吴彩娥,方升佐,冯宗帅,杨万霞,李婷婷,贾韶千.青钱柳叶总黄酮大孔树脂纯化工[J].农业机械学报,2009,40(6):133-137.
.Process of Total Flavonoids in Cyclocarya paliurus Leaves with Macroporous Resins[J].Transactions of the Chinese Society for Agricultural Machinery,2009,40(6):133-137.
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青钱柳叶总黄酮大孔树脂纯化工   [下载全文]
Process of Total Flavonoids in Cyclocarya paliurus Leaves with Macroporous Resins   [Download Pdf][in English]
  
DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]
中文关键词:  总黄酮  青钱柳叶  大孔树脂  纯化
基金项目:
吴彩娥  方升佐  冯宗帅  杨万霞  李婷婷  贾韶千
南京林业大学
中文摘要:为提高青钱柳叶总黄酮的纯度,筛选适宜青钱柳叶总黄酮纯化的大孔树脂,确定青钱柳叶总黄酮纯化工艺参数,选用大孔树脂材料,采用静态与动态吸附—解吸方法对青钱柳叶总黄酮进行纯化,以吸附量与洗脱率为考察指标对树脂进行选择与工艺研究,并利用紫外可见分光光度计测量青钱柳叶总黄酮的含量。结果表明:AB—8型大孔树脂纯化效果最好,其最佳工艺为进样速度2BV/h、进样质量浓度1.0mg/mL,洗脱剂最适宜体积分数与洗脱速度分别为70%和2BV/h。表明AB—8型大孔树脂适合青钱柳叶总黄酮的纯化。
Key Words:
Abstract:With the aim to improve the purity of total flavonoids of Cyclocarya paliurus leaves, the suitable resin for separating and purifying total flavonoids of Cyclocarya paliurus leaves and the optimum parameters of purification were studied. The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests, and the separation techniques were evaluated by measuring the concentration of total flavonoids of Cyclocarya paliurus leaves with ultraviolet spectrophotometer. The results showed that the AB—8 macroporous resin gave the best effect of separation with the sampling rate 2BV/h and concentration 1.0mg/mL, respectively, and the concentration of elution was 70% alcohol, with eluting velocity 2BV/h. In conclusion, AB—8 resin could be applied to purify total flavonoids of Cyclocarya paliurus leaves.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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