香菇多糖复合酶法提取及其脱色工艺优
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Technology on Extraction and Decoloration of Lentinan by Composite Enzyme Method
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    摘要:

    研究了复合酶法提取香菇多糖的最佳工艺条件以及香菇多糖的脱色工艺方法。采用木瓜蛋白酶、纤维素酶复合处理,通过正交试验确定了酶法提取香菇多糖的最佳工艺:木瓜蛋白酶与纤维素酶质量比为2,酶解反应的温度5℃,pH值6.5,反应时间3h,提取率可达16.1%;采用正交试验方法确定了最佳脱色工艺条件为:活性炭用量2%、脱色温度30℃、酶解液pH值4.0、脱色时间90min,多糖损失率和脱色率分别为

    Abstract:

    8.09%和71.21%。The optimum processing conditions of the lentinan enzymatic extraction and the decoloration method of mushroom polysaccharides were discussed. By using papain and cellulose enzyme complex processing method, the ratio of the volume, temperature, the optimum pH value and role of time for orthogonal experiment were determined respectively. The optimum technology of enzymatic extraction of lentinan was as follows: the quality proportion of papain and cellulose enzyme was 2, the enzyme reaction temperature was 55℃, pH value was 6.5, the reaction time was 3h. The extraction rate reached 16.1 percent under this condition. The optimum condition of decoloration technology by orthogonal experiment was as follows:quantity of activated carbon was 2.0%, temperature of decoloration was 30℃,pH value was 4.0, the adsorption time was 90min.

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邹东恢,梁敏,杨勇,王文侠.香菇多糖复合酶法提取及其脱色工艺优[J].农业机械学报,2009,40(3):135-138.

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