3-D synchronization imaging system was developed to realize isochronous acquisition of 3-D images of dairy cow. Distributed control structure has been adopted to correspond orientation device and imaging equipment so that they could work according to schedule. Three CCD color video camera and one DH—QP300 4-channel color frame grabber were adopted to realize isochronous grabbing of 3-D images of the dairy cow. LabVIEW software has been applied to transfer dynamic link libraries to control current frame grabber, single field grabbing mode was adopted to improve image quality. The experimental result showed that the time of images acquisition and linear appraisal is about 3 min, which is less than the standard time 5 min. And the images quality with clear edge and light noise could satisfy the requirements of continued feature extraction algorithm. 