A sort of portable acquisition system about the dynamic parameter of the cool-starting diesel engine was developed. The data acquisition card was used by the system, which contained with start voltage sensor, start electric current sensor, rotate speed sensor and F/V. The real-time collection could be carried out to start voltage of the storage battery, start electric current of the electromotor, temperature of the electrolyte and rotate speed of the diesel engine by multi-channel, and the signals could also be storied, transacted and displayed at the same time. The system could conveniently compile interface and demarcate program by DASYLab. The test results show that the system is reliable, accurate and has good anti-jamming ability. It can meet the needs of the dynamic data acquisition for the cool-starting diesel engine greatly.